2010 Fiscal Year Final Research Report
Physiological role of PAFAH2, an oxidized phospholipid-hydrolyzing enzyme and PAFAH2-derived bioactive lipids
| Project/Area Number |
21790060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KONO Nozomu The University of Tokyo, 大学院・薬学系研究科, 助教 (50451852)
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| Project Period (FY) |
2009 – 2010
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| Keywords | PAF-AH / 酸化リン脂質 |
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| Research Abstract |
In this study, we obtained following results:
(1) Using immunoprecipitation coupled with mass spectrometry, several PAFAH2-interacting proteins including PRDX2 and SCP2 were identified.
(2) From mutational analysis of PAFAH2, Cys-83 was found to be the only residue conferring sensitivity of PAFAH2 to DTNB, suggesting that PAFAH2 activity can be regulated by modification of Cys-83.
(3) An immunocytochemical assay to detect the translocation of PAFAH2 from cytosol to membrane was established.
(4) PAFAH2-deficient mice exhibited reduced PCA reaction most probably by impaired mast cell degranulation.
(5) Bone marrow-derived mast cells from PAFAH2-deficient mice also showed decreased IgE-dependent degranulation.
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