Generation and characterization of Rassf1c knockout mice.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Keio University (2010); Tokyo Medical and Dental University (2009)
• Principal Investigator: NAKAMURA Takashi Keio University, 医学部, 助教 (80431948)
• Research Collaborator: NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
• Project Period (FY): 2009 – 2010
• Keywords: DNA損傷応答 / ストレス応答性キナーゼ / ノックアウトマウス / Rassf1c

Research Abstract

To define the physiological function of Rassf1c protein, we used gene targeting in mouse embryonic stem cells to generate protein Rassf1c knockout mice. The knockout mice showed significant growth retardation. These results suggest that the ability of mice to repair Rassf1c proteins is essential for normal growth.

Research Products

• [Journal Article] Osteoclast-specific Dicer gene deficiency suppresses osteoclastic bone resorption. (2010)
  • Author(s): Mizoguchi F., Izu Y., Hayata T., Hemmi H., Nakashima K., Nakamura T., Kato S., Miyasaka N., Ezura Y., Noda M.
  • Journal Title: J Cell Biochem 109(5) Pages: 866-875
  • Peer Reviewed
• [Journal Article] Combinatory effects of androgen receptor deficiency and hind limb unloading on bone. (2009)
  • Author(s): Saita Y., Nakamura T., Mizoguchi F., Nakashima K., Hemmi H., Hayata T., Ezura Y., Kurosawa H., Kato S., Noda M.
  • Journal Title: Horm Metab Res. 41(11) Pages: 822-828
  • Peer Reviewed
• [Journal Article] Liver development : lessons from knockout mice and mutant fish. (2009)
  • Author(s): Nakamura T., Nishina H.
  • Journal Title: Hepatol Res. 39(7) Pages: 633-644
  • Peer Reviewed
• [Journal Article] A novel microRNA reguratory mechanism of osteoblast differentiation. (2009)
  • Author(s): Inose H., Ochi H., Kimura A., Fujita K., Xu R., Sato S., Iwasaki M., Takeuchi Y., Fukumoto S., Saito K., Nakamura T., Siomi H., Ito H., Arai Y., Shinomiya K-I., Takeda S.
  • Journal Title: Proc Natl Acad Sci USA. 106(49) Pages: 20794-20799
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying the effects of sex steroids on bone and mineral metabolism. (2009)
  • Author(s): Imai Y, Nakamura T, Matsumoto T, Takaoka K, Kato S.
  • Journal Title: J Bone Miner Metab. 27(2) Pages: 127-130
  • Peer Reviewed
• [Journal Article] The antiapoptotic protein Bcl-xL negatively regulates the bone-resorbing activity of osteoclasts in mice. (2009)
  • Author(s): Iwasawa M., Miyazaki T., Nagase Y., Akiyama T., Kadono Y., Nakamura M., Oshima Y., Yasui T., Matsumoto T., Nakamura T., Kato S., Hennighausen L., Nakamura K., Tanaka S.
  • Journal Title: J Clin Invest 119(10) Pages: 3149-3159
  • Peer Reviewed
• [Journal Article] Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. (2009)
  • Author(s): Ohata S, Nawa M, Kasama T, Yamasaki T, Sawanobori K, Hata S, Nakamura T, Asaoka Y, Watanabe T, Okamoto H, Hara T, Terai S, Sakaida I, Katada T, Nishina H.
  • Journal Title: Biochem Biophys Res Commun. 379(4) Pages: 817-823
  • Peer Reviewed
• [Presentation] 骨細胞による骨溶解:授乳期マウス腓骨での骨小腔体積の解析 (2010)
  • Author(s): 松尾光一、武田佳彦、高田康成、中村貴、末松誠、矢代航、南郷脩史、百生敦
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-23
• [Presentation] 破骨細胞分化におけるEpigenetic制御機構の解析 (2010)
  • Author(s): 延〓榮、峯畑健一、高田伊知郎、今井祐記、中村貴、鈴木健之、加藤茂明
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-23
• [Presentation] 質量顕微鏡法を用いた再生肝の解析 (2009)
  • Author(s): 中村貴、井上菜穂子、瀬藤光利、仁科博史
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形
  • Year and Date: 2009-06-26
• [Presentation] 顕微質量分析装置を用いた再生肝の解析 (2009)
  • Author(s): 中村貴、仁科博史
  • Organizer: 第10回日本肝臓医生物学研究会
  • Place of Presentation: 金沢
  • Year and Date: 2009-04-18
• [Remarks] ホームページ等
  • URL: http://www.tmd.ac.jp/mri/dbio/