2010 Fiscal Year Final Research Report

Production of secretory IgA against Shiga-like toxin B subunit using liposomes entrapping MHC-binding peptides

Research Project

Project/Area Number	21790086
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	University of Shizuoka
Principal Investigator	KUROHANE Kohta University of Shizuoka, 薬学部, 助教 (90333525)
Project Period (FY)	2009 – 2010
Keywords	粘膜免疫 / 免疫学 / リポソーム / ワクチン / IgA / 感染症 / ベロ毒素 / 腸管出血 / 性大腸菌
Research Abstract	B subunit of Shiga-like toxin (Stx1B) has been demonstrated to be a poor immunogenicity in mice. To augments the immunogenicity of Stx1B, Stx1B and major histocompatibility complex class II bind peptides, including T cell epitopes were liposomalized. Mice were given Stx1B-liposomes via nasal cavity. As a result, lipsomalization of Stx1B efficiently augmented the immunogenicity of Stx1B in the mucosal immune systems. Moreover, secretory IgA was obtained by incubation of dimeric IgA and recombinant secretory component, or by transcytosis of the dimeric IgA through epithelial cells expressing poly-Ig receptor in vitro.

(4 results)