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2010 Fiscal Year Final Research Report

Analysis of modified low-density lipoproteins in plasma of myocardial infarction

Research Project

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Project/Area Number 21790092
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionShowa University

Principal Investigator

OBAMA Takashi  Showa University, 薬学部, 助教 (60395647)

Co-Investigator(Renkei-kenkyūsha) ITABE Hiroyuki  昭和大学, 薬学部, 教授 (30203079)
KATO Rina  昭和大学, 薬学部, 助教 (30392400)
木庭 新治  昭和大学, 医学部, 講師 (20276546)
KOBAYASHI Yoichi  昭和大学, 医学部, 教授 (70158905)
IWAMOTO Sanju  昭和大学, 医学部, 准教授
MIYAZAKI Akira  昭和大学, 医学部, 教授 (70253721)
Project Period (FY) 2009 – 2010
Keywords動脈硬化症 / 酸化LDL / プロテオーム / モノクローナル抗体 / 酸化ストレス / リポタンパク質 / LDL
Research Abstract

To explore modifications in oxidized low-density lipoprotein (OxLDL) formed in vivo, we analyzed LDL fractions obtained by cardiac catheterization from patients with coronary artery disease. OxLDL was enriched by immunoprecipitation using monoclonal antibody against oxidized phosphatidylcholine (oxPC). Western blot analysis revealed that the OxLDL in vivo was modified by lipid peroxidation products such as 4-hydroxynonenal, acrolein and oxPC, but showed a different pattern from Cu^<2+>-induced OxLDL. These data suggest the structural aspects of oxLDL in vivo different from in vitro oxidation.

  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] The dynamics of oxidized LDL during atherogenesis2011

    • Author(s)
      Itabe H, Obama T, Kato R
    • Journal Title

      Journal of Lipids Article ID

      Pages: 418313

    • Peer Reviewed
  • [Journal Article] Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver.2011

    • Author(s)
      Takahashi K, Sasabe N, Ohshima K, Kitazato K, Kato R, Masuda Y, Tsurumaki M, Obama T, Okudaira S, Aoki J, Arai H, Yamaguchi T, Itabe H.
    • Journal Title

      J lipid Res. 51

      Pages: 2571-2580

    • Peer Reviewed
  • [Journal Article] Immunoprecipitation and LC-MS/MS analysis of human oxidized low-density lipoprotein from plasma2010

    • Author(s)
      Obama T, Kikuchi M, Shiraiwa T, Kato R, Itabe H
    • Journal Title

      Chemistry and Physics of Lipids 163S

      Pages: S42

    • Peer Reviewed
  • [Journal Article] Oxidized Low-Density Lipoproteins enhances IL-8 production in Human Gingival Epithelial Cells2010

    • Author(s)
      Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M
    • Journal Title

      J Period Res. 45

      Pages: 488-495

    • Peer Reviewed
  • [Journal Article] Transient Increase in Plasma Oxidized LDL During the Progression of Atherosclerosis in Apolipoprotein E Knockout Mice.2009

    • Author(s)
      Kato R, Mori C, Kitazato K, Arata S, Obama T, Mori M, Takahashi K, Aiuchi T, Takano T, Itabe H.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 29

      Pages: 33-39

    • Peer Reviewed
  • [Presentation] 生体内で生じた酸化LDLの脂質過酸化物による修飾2011

    • Author(s)
      小濱孝士、白岩誉裕希、楠ノ瀬雄也、加藤里奈、山口智広、板部洋之
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
  • [Presentation] Immunoprecipitation and LC-MS/MS analysis of human oxidized low-density lipoprotein from plasma2010

    • Author(s)
      Obama T, Kikuchi M, Shiraiwa T, Kato R, Itabe H
    • Organizer
      51st International Conference on the Bioscience of Lipids
    • Place of Presentation
      Bilbao, Spain
    • Year and Date
      2010-09-09
  • [Presentation] 動脈硬化の発症と大動脈組織の脂質過酸化物沈着2010

    • Author(s)
      林暢孝、加藤里奈、宮越由佳、天日雅行、小濱孝士、山口智広、板部洋之
    • Organizer
      第11回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-06-19
  • [Presentation] apoEノックアウトマウス大動脈の動脈硬化初期段階における脂質過酸化物の出現2010

    • Author(s)
      林暢孝、加藤里奈、宮越由佳、天日雅行、荒田悟、小濱孝士、山口智広、板部洋之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-29
  • [Presentation] 肝トリグリセリド合成の制御機構の解析~コレステロール摂取によるlipin発現の抑制2009

    • Author(s)
      小濱孝士、赤木和貴、長岡彩香、堀内尚美、加藤里奈、山口智広、板部洋之
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      群馬
    • Year and Date
      2009-06-04
  • [Remarks] ホームページ等

    • URL

      http://www10.showa-u.ac.jp/~biolchem/

  • [Patent(Industrial Property Rights)] タンパク質又はペプチドのスルホン酸誘導体化されたN末端ペプチドフラグメントを質量分析する方法2010

    • Inventor(s)
      乗岡茂巳、山口実、九山浩樹、小濱孝士、安藤英治、中澤隆、上山憲一、岡村高明
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      特許,特許第4576972号
    • Acquisition Date
      2010-09-03
  • [Patent(Industrial Property Rights)] タンパク質又はペプチドをスルホン酸誘導体化する方法、及びタンパク質又はペプチドのアミノ酸配列を決定する方法2010

    • Inventor(s)
      小濱孝士、山口実、九山浩樹、安藤英治
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      特許,特許第4569236号
    • Acquisition Date
      2010-08-20
  • [Patent(Industrial Property Rights)] Methods for derivatizing protein or peptide with sulfonic acid groups2009

    • Inventor(s)
      乗岡茂巳、山口実、九山浩樹、小濱孝士、安藤英治、中澤隆、上山憲一、岡村高明
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      United States Patent,7,579,165
    • Acquisition Date
      2009-08-25

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Published: 2012-02-13   Modified: 2016-04-21  

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