ER cholesterol regulates the biogenesis of LD and the ApoB secretion

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790105
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MAKINO Asami The Institute of Physical and Chemical Research, 小林脂質生物学研究室, 特別研究員 (20373368)
• Project Period (FY): 2009 – 2010
• Keywords: 脂肪滴 / 小胞体 / コレステロール / VLDL / ACAT / ADRP / Rab18 / ApoB

Research Abstract

Cholesterol loading in ER induced the enlargement of lipid droplets and the degradation of major LD protein, adipose differentiation-related protein (ADRP) in cultured human hepatocytes. However, lipid particles were not disappeared. Instead, the lipids were surrounded by VLDL protein, ApoB. This step was controlled by rab18. These results indicate that cholesterol modulates both biogenesis/maintenance of LDs and ApoB secretion.

Research Products

• [Journal Article] Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. (2011)
  • Author(s): Makino A, 9名中4番目
  • Journal Title: Chemical Science. (in press)
  • Peer Reviewed
• [Journal Article] Multiplex analysis of sphingolipids using amine-reactive tags (iTRAQ). (2011)
  • Author(s): Makino A, 9名中2番目
  • Journal Title: J Lipid Res. 52(6) Pages: 1294-1302
  • Peer Reviewed
• [Journal Article] Novel lipogenic enzyme ELOVL7 is involved in prostate cancer growth through saturated long-chain fatty acid metabolism. (2009)
  • Author(s): Makino A, 12名中2番目
  • Journal Title: Cancer Res. 69(20) Pages: 8133-8140
  • Peer Reviewed
• [Presentation] Endoplasmic reticulum cholesterol controls both ApoB secretion and the formation of lipid droplets (2011)
  • Author(s): Makino A
  • Organizer: RIKEN-SNU (Seoul National University) workshop on Chemistry and Biology
  • Place of Presentation: Saitama
  • Year and Date: 20110303-20110304
• [Presentation] Visualization of lipids and lipid domains (2011)
  • Author(s): Kobayashi T, Abe M, Kishimoto T, Makino A, Hullin-Matsuda F, Ishitsuka R, Murate M
  • Organizer: 第53回日本脂質生化学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] 「小胞体コレステロールによる脂肪滴形成とリポタンパク質分泌の制御」文部科学省科学研究費補助金「新学術領域研究」 (2010)
  • Author(s): 小林俊秀、牧野麻美
  • Organizer: 第二回「細胞内ロジスティクス」班会議
  • Place of Presentation: 札幌
  • Year and Date: 20100628-20100701
• [Presentation] 肝細胞における小胞体コレステロールによる脂肪滴形成とリポタンパク質分泌の制御 (2010)
  • Author(s): 牧野麻美
  • Organizer: 理研バイオアーキテクトシンポジウム2010
  • Place of Presentation: 熱海
  • Year and Date: 20100224-20100226
• [Presentation] Endoplasmic reticulm chlesterol controls both ApoB section and the formation of lipid droplets in cultured hepatocytes (2010)
  • Author(s): Makino A.
  • Organizer: 13th International Membrane Research Forum/6th iCeMS International Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100127-20100129
• [Presentation] Screening of new inhibitors of sphingolipid metabolism. 10eme Journee Scientifique (2010)
  • Author(s): Hullin-Matsuda F, Ishii K, Makino A, Ishitsuka R, Lagarde M, Vidal H, Kobayashi T
  • Organizer: Institut Mutidisciplinaire de Biochimie des Lipides, Juin.21
  • Place of Presentation: Lyon, France
  • Year and Date: 20100000
• [Presentation] Imaging lipids and lipid domains (2009)
  • Author(s): Kobayashi T, Abe M, Ishitsuka R, Shimada Y, Makino A, Murate M
  • Organizer: The First International Symposium on Membrane Biology.
  • Place of Presentation: Guilin, China
  • Year and Date: 20090709-20090711
• [Presentation] Lipid rafts : new tools, new fluorescent probes 6^<th> Proceedings of the GERLI Lipidomics Meeting. (2009)
  • Author(s): Kobayashi T, Abe M, Ishitsuka R, Shimada Y, Makino A, Murate M
  • Organizer: Bioactive Lipids, Nutrition and Health.
  • Place of Presentation: Rennes, France
  • Year and Date: 20090701-20090703
• [Presentation] The late endsome lipid, bis(monoacylglycero)phosphate modifies the metabolism and transport of cellular cholesterol (2009)
  • Author(s): Hullin-Matsuda F, Bouvier J, Delton-Vandenbroucke I, Makino A, Lagarde M, Kobayashi T.
  • Organizer: 4th International conference on phospholipase A2 and lipid mediators(PLM2009)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20090525-20090528
• [Presentation] Visualization of lipid domains (2009)
  • Author(s): 小林俊秀, 阿部充宏, 石塚玲子, 嶋田有紀子, 牧野麻美, 村手源英
  • Organizer: 第59回FCCAセミナー/環境医学研究所ワークショップ2009
  • Place of Presentation: 東京
  • Year and Date: 2009-10-20
• [Presentation] Imaging lipid domains (2009)
  • Author(s): Kobayashi T, Abe M, Ishitsuka R, Shimada Y, Makino A, Murate M
  • Organizer: 22^nd Biennial Meeting of the ISN/APSN Joint Meeting.
  • Place of Presentation: Busan, Korea
  • Year and Date: 2009-08-27
• [Presentation] Limonoid ; a family of phytocompounds as new inhibitors of sphingolipid metabolism (2009)
  • Author(s): Hullin-Matsuda F, Ishii K, Makino A, Ishitsuka R, Kobayshi T
  • Organizer: Inserm-Riken Lipidmics Unit (IRLU) 1st Meeting
  • Place of Presentation: Lyon, France
  • Year and Date: 2009-06-18
• [Presentation] Endoplasmic reticulum cholesterol controls both ApoB secretion and the formation of lipids droplets in cultured hepatocytes (2009)
  • Author(s): Makino A, Hullin-Matsuda F, Murate M, Abe M, Delton-Vandenbroucke I, Lagarde M, Kobayashi T
  • Organizer: Inserm-Riken Lipidmics Unit (IRLU) 1st Meeting
  • Place of Presentation: Lyon, France
  • Year and Date: 2009-06-18
• [Presentation] Delton-Vandenbroucke I. Regulation of cellular cholesterol homeostasis by bismonoacylglycerophosphate, an endosome-specific phospholipid (2009)
  • Author(s): Bouvier J, Makino A, Hullin-Matsuda F, Lagarde M, Kobayashi T
  • Organizer: Inserm-Riken Lipidmics Unit (IRLU) 1st Meeting
  • Place of Presentation: Lyon, France
  • Year and Date: 2009-06-18
• [Patent(Industrial Property Rights)] 新規なタンパク質およびその利用 (2010)
  • Inventor(s): 小林俊秀(発明協力者牧野麻美、阿部充宏、石塚玲子、村手源英)
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 1,特願2010-112681
  • Filing Date: 2010-05-14