2010 Fiscal Year Final Research Report
The role of the transcriptional regulator, Ferric uptake regulator (Fur), in H.pylori resistance to metronidazole
| Project/Area Number |
21790133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Keio University |
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Principal Investigator |
TSUGAWA Hitoshi Keio University, 医学部, 助教 (30468483)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 薬剤耐性 / 酸化ストレス / ferric uptake regulator(Fur) / superoxide dismutase(SOD) |
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| Research Abstract |
Metronidazole (Mtz) is a prodrug that is converted to its active form and superoxide radicals are generated. The transcriptional regulator, ferric uptake regulator (Fur), of H.pylori is a direct suppressor of the ironcofactored superoxide dismutase (SodB), which is essential for protection against superoxide attack. In some Mtz-resistant strains, SodB activity is induced in a dose-dependent manner on exposure to Mtz. These Mtz-resistant strains were found to carry amino acids mutation of Fur (C78Y, P114S ; mutant-type Fur). The binding affinity of the mutant-type Fur to the sodB promoter (Fur-Box) was significantly reduced. Our approach demonstrated that SodB expression is derepressed by mutant-type Fur, which is associated with the development of Mtz resistance.
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[Presentation] The iron-uptake system for the antioxidant ability of Helicobacter pylori.2011
Author(s)
Tsugawa, H., Suzuki, H., Hirata, K., Matsuzaki, J., Okada, S., Fukuhara, S., Saito, Y., Hibi, T.
Organizer
International Symposium on Free Radical Research ; Contribution to Medicine(Poster Session)
Place of Presentation
Kyoto
Year and Date
2011-01-21
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[Presentation] The role of the Ferric uptake regulator (Fur) in Helicobacter pylori resistance to metronidazole.2010
Author(s)
Tsugawa, H., Suzuki, H., Suzuki, S., Hirata, K., Matsuzaki, J., Saito, Y., Hibi, T.
Organizer
Digestive Disease Week 2010, Topic forum(Oral Session)
Place of Presentation
New Orleans, USA
Year and Date
2010-05-04
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[Presentation] Induction of H.pylori resistance to metronidazole through enhanced expression of a bacterial efflux pump.2010
Author(s)
Tsugawa, H., Suzuki, H., Muraoka, H., Suzuki, S., Hirata, K., Matsuzaki, J., Saito, Y., Hibi, T.
Organizer
Digestive Disease Week 2010, Research forum(Oral Session)
Place of Presentation
New Orleans, USA
Year and Date
2010-05-02
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[Presentation] Mutation in the transcriptional repressor Fur contributes to the mechanism of metronidazole resistance of Helicobacter pylori ; implication of the structural-functional relationships of mutant-type Fur.2009
Author(s)
Tsugawa, H., Suzuki, H., Hirata K., Satoh, K., Hibi, T.
Organizer
15^<th> International Workshop on Campylobacter, Helicobacter, and Related organisms (CHRO2009)(Poster Session)
Place of Presentation
Niigata, Japan
Year and Date
2009-09-04
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[Presentation] Ferric uptake regulator (Fur) mutants of Helicobacter pylori associated with the development of resistance to Metronidazole : implications for the structural-functional relationships of mutant-type Fur.2009
Author(s)
Tsugawa, H., Suzuki, H., Nakagawa, I., Hirata K., Nishizawa, T., Saito, Y., Juntaro M., Eisuke I., Suematsu, M., Hibi, T.
Organizer
Digestive Disease Week 2009, Research forum(Oral Session)
Place of Presentation
Chicago, USA
Year and Date
2009-05-31
