2010 Fiscal Year Final Research Report
Development of tissue specific siRNA expression system to vascular endothelial cells in cancer
| Project/Area Number |
21790161
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Medical pharmacy
|
|---|
| Research Institution | Showa Pharmaceutical University |
|---|
Principal Investigator |
KOIZUMI Naoya Showa Pharmaceutical University, 薬学部, 助教 (80433845)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | 医療薬剤学 / 癌遺伝子治療 / RNAi / ターゲティング / 血管内皮細胞 |
|---|
| Research Abstract |
We tried development of novel tissue specific siRNA expression system for cancer gene therapy by VSV-G lentivirus vector. Therefore, we substituted sequence of CCR5-siRNA in endogenous microRNA for driving by tissue specific promoter (RNA polymerase type II). As a result, the siRNA expression system had not worked by RNA polymerase type II promoter. We need further study for the development of tissue specific siRNA expression system.
|
-
[Journal Article] A highly efficient short hairpin RNA potently down-regulates CCR5 expression in systemic lymphoid organs in the hu-BLT mouse model.2010
Author(s)
Shimizu S, Hong P, Arumugam B, Pokomo L, Boyer J, Koizumi N, Kittipongdaja P, Chen A, Bristol G, Galic Z, Zack JA, Yang O, Chen IS, Lee B, An DS.
-
Journal Title
Peer Reviewed
-
