Immunohistological analysis of VAMP5 in skeletal muscle

2011 Fiscal Year Final Research Report

• Project/Area Number: 21790175
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Gunma University
• Principal Investigator: TAJIKA Yuki 群馬大学, 大学院・医学系研究科, 助教 (90400738)
• Project Period (FY): 2009 – 2011
• Keywords: 骨格筋 / 小胞輸送

Research Abstract

Membrane trafficking is a fundamental event for the life of cell. Previous studies using neuron and epithelial cells revealed its molecular mechanism, including SNARE proteins, which regulate membrane tethering and fusion process. VAMP5, a member of SNARE proteins, was found in transcripts from skeletal muscle, however, the protein levels in a whole body was not known. In this study, we performed immunostaining on skeletal muscle and other tissues, and clarified the expression pattern of VAMP5.

Research Products

• [Journal Article] VAMP2 marks quiescent satellite cells and myotubes, but not activated myoblasts (2010)
  • Author(s): Tajika Y, Takahashi M, Hino M, Murakami T, Yorifuji H
  • Journal Title: Acta Histochemica et Cytochemica Volume: Vol.43 (4) Pages: 107-114
  • Peer Reviewed
• [Journal Article] Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells (2010)
  • Author(s): Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H.
  • Journal Title: Journal of Electron Microscopy Volume: Vol.59 (6) Pages: 495-501
  • Peer Reviewed
• [Journal Article] Insufficient membrane fusion in dysferlin-deficient muscle fibers after heavy-ion irradiation (2009)
  • Author(s): Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Kobayashi Y, Yorifuji H
  • Journal Title: Cell Structure and Function Volume: Vol.34 Pages: 11-15
  • Peer Reviewed
• [Presentation] 骨格筋細胞におけるSNARE蛋白質の発現パターン (2011)
  • Author(s): 多鹿友喜, 高橋麻衣子, Astrid F. K., 上野仁之, 村上徹, 依藤宏
  • Organizer: 第52回日本組織細胞化学会
  • Place of Presentation: 金沢大学
  • Year and Date: 2011-09-24
• [Presentation] 骨格筋におけるVAMP5の発現レベルは、線維タイプにより異なる (2011)
  • Author(s): 高橋麻衣子, 多鹿友喜, Astrid F. K., 日野瑞城, 上野仁之, 村上徹, 依藤宏
  • Organizer: 第116回日本解剖学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-03-30
• [Presentation] 骨格筋細胞に特徴的な膜小胞輸送システム (2011)
  • Author(s): 多鹿友喜, 高橋麻衣子, Astrid F. K., 上野仁之, 村上徹, 依藤宏
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学
  • Year and Date: 2011-03-27
• [Presentation] 膜融合に関与するVAMP5のマウス諸器官における発現と分布 (2011)
  • Author(s): 高橋麻衣子, 多鹿友喜, Astrid F. K., 上野仁之, 村上徹, 依藤宏
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学
  • Year and Date: 2011-03-27
• [Presentation] 筋衛星細胞の活性化過程におけるVAMP2の発現変化 (2010)
  • Author(s): 多鹿友喜, 高橋麻衣子, 佐藤真人, 村上徹, 依藤宏
  • Organizer: 第115回日本解剖学会岩手県民会館
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] 筋ジストロフィーマウス骨格筋におけるVAMP5の発現 (2010)
  • Author(s): 高橋麻衣子, 多鹿友喜, 佐藤真人, 村上徹, 依藤宏
  • Organizer: 第115回日本解剖学会岩手県民会館
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] 小胞輸送関連タンパクVAMP2の細胞内分布に関する組織化学的検討 (2009)
  • Author(s): 多鹿友喜, 佐藤真人, 村上徹, 依藤宏
  • Organizer: 第56回北関東医学会総会
  • Place of Presentation: 群馬大学刀城会館
  • Year and Date: 2009-10-08
• [Remarks]
  • URL: http://anatomy.dept.med.gunma-u.ac.jp/~taji/