2010 Fiscal Year Final Research Report
Three-dimensional structural and functional analysis of the Golgi apparatus by new method of scanning electron microscopy
| Project/Area Number |
21790176
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Niigata University |
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Principal Investigator |
KOGA Daisuke 新潟大学, 医歯学系, 助教 (30467071)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ゴルジ装置 / 細胞小器官 / 下垂体前葉 / 走査型電子顕微鏡 / オスミウム浸軟法 / ポリエチレングリコール / 試料作製法 |
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| Research Abstract |
A novel high resolution three-dimensional observation method was developed by an effective combination of light microscopic technique and scanning electron microscopic observation. This method was especially useful for investigation of tissues containing various kinds of cell, enabling the classification of anterior pituitary cells which has hitherto been difficult by using scanning electron microscopy. Furthermore, the three-dimensional ultrastructure in relation to its function of each anterior pituitary cell of the Golgi apparatus was precisely analyzed by high-resolution scanning electron microscopy.
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