2010 Fiscal Year Final Research Report
Analysis for cell polarity regulation by using drug inducible gene-knock-out system.
| Project/Area Number |
21790177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKAYA Masa-aki Kanazawa University, 医学系, 助教 (70422095)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 細胞極性 / Cre-loxP / 発生生物学 / 形態形成学 / 分子細胞生物学 |
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| Research Abstract |
In this study, we constructed novel experimental system, which enable us to disrupt gene function under the control of Tamoxifen by using Cre-loxP technology. We focused on two polarity related genes, aPKC(atypical Protein kinase C) and Daam1(Deshevelled associated activator of morphogenesis), and successfully detected genetic interaction and cellular phenotype. Further, we confirmed this results by using Xenopus injection experiment and found the possibility that aPKC acts as down stream of Daam1.
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