2010 Fiscal Year Final Research Report
Regulation of L-type Ca^<2+> channel activity by calmodulin
| Project/Area Number |
21790206
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
MINOBE Etsuko Kagoshima University, 医歯学総合研究科, 助教 (00448581)
|
|---|
| Research Collaborator |
MORI Masayuki 福岡大学, 医学部, 講師 (80342640)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | カルシウムチャネル / カルモジュリン / パッチクランプ法 / inside-out / run-down / カルシウムイオン / ATP |
|---|
| Research Abstract |
We measured activity of L-type Ca^<2+> channel and its mutants, C-terminal deletion (delta CT) and delta CT linked with calmodulin (CaM), using patch-clamp technique (inside-out mode). We found that binding of CaM to the channel was required for the channel activity. Unlike the wild type channel, the mutants did not require ATP for the channel activity. It was suggested that CaM and ATP interacted with a proximal part of C-terminal thereby producing the channel activity, which was modulated negatively by a distal part of C-terminal.
|
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Regulation of Cav1.2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII.2009
Author(s)
Masaki Kameyama, Li-ying Hao, Etsuko Minobe, Jian-jun Xu, Dong-yun Han, Hong-guang Nie, Zahangir A.Saud, Wu-yang Wang, Hadhimulya Asmara, Kazuto Yazawa.
Organizer
The IUPS 2009 satellite symposium.
Place of Presentation
京都
Year and Date
20090802-20090804
-
-
-
-
-
[Presentation] Ca^<2+> channels and cardiac functions.2009
Author(s)
Masaki Kameyama, Li-ying Hao, Etsuko Minobe, Jian-jun Xu, Dong-yun Han, Hong-guang Nie, Zahangir A.Saud, Wu-yang Wang, Hadhimulya Asmara.
Organizer
International Congress of Physiological Sciences.
Place of Presentation
京都
Year and Date
20090727-20090801
-
