2010 Fiscal Year Final Research Report
Identification of the anion channel involved in volume regulation in the principal cells of rat cortical collecting duct.
| Project/Area Number |
21790212
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KOMAGIRI You Iwate Medical University, 医学部, 助教 (80405753)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 生体膜 / チャネル / トランスポーター / 能動輸送 |
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| Research Abstract |
Anion efflux and intracellular Ca^<2+> increase have been known to play important roles in cell volume regulation during hypotonicity. This study was carried out to explore the mechanisms of hypotonicity-induced intracellular Ca^<2+> elevation and characterize anion conductance activated by hypotonicity in the principal cells of freshly isolated rat cortical collecting ducts. Using Fura-2 fluorescence Ca^<2+> imaging and whole-cell voltage-clamp techniques, it was found that a nicardipine-sensitive Ca^<2+> entry pathway was involved in the mechanisms underlying the hypotonicity-induced intracellular Ca^<2+> increase and a NPPB-sensitive/glibenclamide-insensitive Cl^- current was activated by hypotonic stimulation in the principal cells of rat cortical collecting ducts.
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