2010 Fiscal Year Final Research Report

The identification of a novel mechanism of osteoclast differentiation that is calcium oscillation-independent osteoclastogenesis

Research Project

Project/Area Number	21790220
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General physiology
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	KURODA Yukiko The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 基礎科学特別研究員 (70455343)
Project Period (FY)	2009 – 2010
Keywords	分化 / 破骨細胞 / カルシウムオシリーション / 細胞融合
Research Abstract	By analysis of osteoclasts derived from IRBIT knockout mice, we found that the number of RANKL-induced Ca^<2+> oscillation positive cells was increased in IRBIT knockout mononuclear osteoclasts and that IRBIT knockout cells enhanced cell-cell fusion step during osteoclastogenesis as compared with wild-type cells. These results indicate that IRBIT negatively regulates IP_3R-mediated Ca^<2+> signaling and cell-cell fusion step during osteoclast differentiation.