Visualization of stress reaction in neuroendocrine system using multiple fluorescent protein transgenic rats.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: The University of Tokushima (2010); University of Occupational and Environmental Health, Japan (2009)
• Principal Investigator: FUJIHARA Hiroaki The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (10369051)
• Project Period (FY): 2009 – 2010
• Keywords: トランスジェニック / ラット / RFP / ストレス

Research Abstract

I examined the effect of osmotic stimulus using c-fos-mRFP transgenic rats, c-fos-mRFP/arginine vasopressin-eGFP double transgenic rats and oxytocin-mRFP transgenic rats. In all transgenic rats, fluorescence and mRNA levels of the c-fos-mRFP, arginine vasopressin-eGFP and oxytocin-mRFP were increased after osmotic stimulus. I succeeded in visualization of stress reaction in neuroendocrine system as index of green fluorescence and red fluorescence.

Research Products

• [Journal Article] Specific expression of an oxytocin- enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary. (2010)
  • Author(s): Katoh A, Fujihara H, Ohbuchi T, Onaka T, Young WS 3rd, Dayanithi G, Yamasaki, Kawata M, Suzuki H, Otsubo H, Suzuki H, Murphy D, Ueta Y.
  • Journal Title: Journal of Endocrinology 204(3) Pages: 275-285
  • Peer Reviewed
• [Journal Article] Induction of the arginine vasopressin- enhanced green fluorescent protein fusion transgene in the rat locus coeruleus. (2010)
  • Author(s): Todoroki M, Ueta Y, Fujihara H, Otsubo H, Shibata M, Hashimoto H, Kobayashi M, Sakamoto H, Kawata M, Dayanithi G, Murphy D, Hiro H, Takahashi K, Nagata S.
  • Journal Title: Stress 13 Pages: 281-291
  • Peer Reviewed
• [Journal Article] Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus. (2010)
  • Author(s): Maruyama T, Ohbuchi T, Fujihara H, Shibata M, Mori K, Murphy D, Dayanithi G, Ueta Y.
  • Journal Title: Peptides 31(11) Pages: 2089-2093
  • Peer Reviewed
• [Journal Article] Highly Visible Expression of an Oxytocin-Monomeric Red Fluorescent Protein 1 Fusion Gene in the Hypothalamus and Posterior Pituitary of Transgenic Rats. (2010)
  • Author(s): Katoh A, Fujihara H, Ohbuchi T, Onaka T, Hashimoto T, Kawata M, Suzuki H, Ueta Y.
  • Journal Title: Endocrinology (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Exaggerated response of a vasopressin- enhanced green fluorescent protein transgene to nociceptive stimulation in the rat. (2009)
  • Author(s): Suzuki H, Kawasaki M, Ohnishi H, Otsubo H, Ohbuchi T, Katoh A, Hashimoto H, Yokoyama T, Fujihara H, Dayanithi G, Murphy D, Nakamura T, Ueta Y.
  • Journal Title: The Journal of Neuroscience 29(42) Pages: 13182-13189
  • Peer Reviewed
• [Journal Article] Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat. (2009)
  • Author(s): Fujihara H, Ueta Y, Suzuki H, Katoh A, Ohbuchi T, Otsubo H, Dayanithi G, Murphy D.
  • Journal Title: Endocrinology 150 Pages: 5633-5638
  • Peer Reviewed
• [Presentation] オキシトシンニューロンにおける赤色蛍光タンパク遺伝子発現 (2011)
  • Author(s): 藤原広明
  • Organizer: 第2回日本病態生理学会サテライトセミナー
  • Place of Presentation: 神戸市北区
  • Year and Date: 2011-01-22
• [Presentation] c-fos-RFPとAVP-eGFPのダブルトランスジェニックラットの作出と浸透圧刺激に対する反応 (2010)
  • Author(s): 藤原広明
  • Organizer: 第20回日本病態生理学会大会
  • Place of Presentation: 奈良県橿原市
  • Year and Date: 2010-01-24