2011 Fiscal Year Final Research Report
Analysis of mechanisms of ERK MAP kinase phosphorylation
| Project/Area Number |
21790273
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
AOKI Kazuhiro 京都大学, 大学院・生命科学研究科, 講師 (80511427)
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| Project Period (FY) |
2009 – 2011
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| Keywords | FRET / イメージング / シグナル伝達 / システム生物学 / 癌 |
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| Research Abstract |
ERK MAP kinase is essential for cell proliferation, differentiation and tumorigenetsis. We analyzed molecular mechanisms of ERK phosphorylation by using imaging and computer simulation. Approximately 30 kinetic parameters were measured by experiments. Based on these empirical parameters, we built a simulation model, and found that ERK was processively, but not distributively, phosphorylated by MEK.
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