Mechanism for interaction between gp91phox and p22phox : two membrane-integrated proteins of the phagocyte NADPH oxidase

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790283
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: MIYANO Kei Kyushu University, 医学研究院, 学術研究員 (60444783)
• Project Period (FY): 2009 – 2010
• Keywords: NADPHオキシダーゼ / 活性酸素 / Nox2 / gp91^<phox> / 膜タンパク質

Research Abstract

The phagocyte NADPH oxidase, dormant in resting cells, is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The catalytic core of the phagocyte oxidase is Nox2/gp91^<phox>, a membrane-spaning protein that forms a stable heterodimer with p22^<phox>. In this study, I found that both N- and C-terminal regions play a crucial role in Nox binding to p22^<phox>. Furthermore, I showed that a short region between the TPR and activation domains in p67^<phox> is crucial for activation of Nox2.

Research Products

• [Journal Article] Sumimoto H Assessment of the role for Rho family GTPase in NADPH oxidase activation Methods (2011)
  • Author(s): Miyano K
  • Journal Title: Mol.Biol. in press
  • Peer Reviewed
• [Journal Article] A conserved region between the TPR and activation domains of p67^phox participates in activation of the phagocyte NADPH oxidase (2010)
  • Author(s): Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H
  • Journal Title: J Biol Chem. 285 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] Membrane phospholipid metabolism during phagocytosis in human neutrophils (2010)
  • Author(s): Minakami R, Maehara Y, Kamakura S, Kumano O, Miyano K, Sumimoto H
  • Journal Title: Genes Cells. 15 Pages: 409-424
  • Peer Reviewed
• [Journal Article] 酸化ストレス応答とNoxファミリー (2009)
  • Author(s): 宮野佳, 住本英樹
  • Journal Title: 生物資料分析 32 Pages: 289-296
  • Peer Reviewed
• [Journal Article] The insert region of the Rac GTPases is dispensable for activation of superoxide-producing NADPH oxidases. (2009)
  • Author(s): Miyano K, Koga H, Minakami R, Sumimoto H
  • Journal Title: Biochem J. 422 Pages: 373-382
  • Peer Reviewed
• [Journal Article] The domain organization of p67^phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. (2009)
  • Author(s): Yuzawa S, Miyano K, Honbou K, Inagaki F, Sumimoto H
  • Journal Title: J Innate Immun. 1 Pages: 543-555
  • Peer Reviewed
• [Presentation] 活性酸素生成型NADPHオキシダーゼ(Nox)ファミリーの活性化における膜貫通領域と細胞質領域の役割 (2010)
  • Author(s): 宮野佳, 住本英樹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] 生体防御に重要な活性酸素生成酵素NADPHオキシダーゼの活性化機構 (2010)
  • Author(s): 宮野佳, 住本英樹
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台
  • Year and Date: 20100722-20100724
• [Presentation] Roles for the N-terminal transmembrane and C-terminal cytosolic moieties of Nox-family NADPH oxidases (2010)
  • Author(s): Miyano K, Sumimoto H
  • Organizer: Gordon Research Conference(Nox family NADPH oxidases)
  • Place of Presentation: Les Diablerets, the Switzerland
  • Year and Date: 20100606-20100611
• [Presentation] The domain organization of p67^phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes (2009)
  • Author(s): 湯澤聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Rac-dependent activation of superoxide-producing NADPH oxidases does not require its insert region (2009)
  • Author(s): 宮野佳, 古賀博文, 水上令子, 住本英樹
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Nox2活性化におけるp67^phoxのドメイン配置と作用機構 (2009)
  • Author(s): 湯澤聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
  • Organizer: 第82回日本生化学会大会
  • Year and Date: 20091021-20091024
• [Presentation] Racのinsert regionは活性酸素生成酵素NAPDHオキシダーゼ活性化に必要ない (2009)
  • Author(s): 宮野佳, 古賀博文, 水上令子, 住本英樹
  • Organizer: 第82回日本生化学会大会
  • Year and Date: 20091021-20091024
• [Presentation] スーパーオキシド生成酵素NADPHオキシダーゼの活性化因子p67^phoxの作用機構の解明 (2009)
  • Author(s): 宮野佳, 前原優一, 湯澤聡, 住本英樹
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Year and Date: 20090516-20090517
• [Book] Front. Gastrointest.Res (2011)
  • Author(s): Sumimoto H, Minakami R, Miyano K
  • Total Pages: 23-346
  • Publisher: In free radical biology in digestive diseases(The Nox Family of NADPH oxidases that Deliberately Produce Reactive Oxygen Species)(S.Karger)