2010 Fiscal Year Final Research Report
Analysis of mRNP complex using gene knockin in mammalian cells
| Project/Area Number |
21790289
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
YAMASHITA Akio Yokohama City University, 医学研究科, 講師 (20405020)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 細胞医化学 |
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| Research Abstract |
I cloned genomic regions of VEGF, IL6, IL5, IFNbeta and IFNgamma. I constructed IFNbeta gene knockin vector for the insertion of BoxB sequence at downstream of termination codon. Next, I generate cell lines (HeLa TetOff, HeLaTetOnAdvanced, HCT116、BEAS-2B TetOff) which containing a single FRT recombination site. I also constructed the pTRE-Tight-FRT IL-6, IFNbeta, IL28B, and IL-5 full length genes.
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[Journal Article] AAA+ family ATPases, RUVBL1 and RUVBL2, coordinate PIKK family and play critical roles in Nonsense-mediated mRNA decay.2010
Author(s)
Izumi N, Yamashita A (corresponding author), Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
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Journal Title
Science signaling (Science姉妹誌). (3)
Pages: 27
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[Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.2009
Author(s)
Yamashita A (corresponding author), Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Kurata R, Hachiya T, Hirano H, Anderson P, Ohno S.
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Journal Title
Genes and Development. 23(9)
Pages: 1091-1105
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[Presentation] THE REMODELING OF MRNA SURVEILLANCE COMPLEX DURING NONSENSE-MEDIATED MRNA DECAY.2009
Author(s)
Yamashita A, Izumi N, Okada-Katsuhata Y, Kutsuzawa K, Muramatsu R, Sarri B, Iwamatsu A, Hirano H, Hirahata F, Anderson P, Ohno S.
Organizer
Cold spring harbor laboratory meeting 2009 mRNA processing
Place of Presentation
New York
Year and Date
20090800
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