2011 Fiscal Year Final Research Report
analysis of human amniotic membrane-derived cells related to hematopoiesis-supportive functions
| Project/Area Number |
21790297
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
SUDO Kazuhiro 独立行政法人理化学研究所, 細胞材料開発室, 協力研究員 (10392002)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 羊膜細胞 / 臍帯細胞 / 造血幹細胞 / 造血支持能 / 共培養 / 共移植 |
|---|
| Research Abstract |
Co-culture of cord blood CD34^+cells with human amniotic membrane(HAM)-or human umbilical cord(HUC)-derived cells caused increase of CD45^+hematopoietic cells. however, percentage of CD34^+cells was decreased with time of co-culture. Colony-forming cells(CFCs) were maintained in co-culture at least for 5 weeks. The chimerisms of human CD45^+cells in NOD/SCID mice were significantly higher when HAM-or HUC-derived cells were co-transplanted with CD34+cells than when CD34^+cells were transplanted alone. Those results strongly suggest that HAM-and HUC-derived cells have hematopoiesis-supportive functions.
|
