Functional analysis of miRNAs and ncRNAs identified by global screening of AR biding sites in prostate cancer cells.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790305
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAYAMA Kenichi The University of Tokyo, 医学部附属病院, 特任臨床医 (50508075)
• Project Period (FY): 2009 – 2010
• Keywords: 癌 / ゲノム / ホルモン

Research Abstract

We tried to identify androgen-regulated miRNAs and ncRNAs(Non-coding RNAs) in prostate cancer cell. First, We found several miRNAs are situated in the vicinity of AR binding sites we identified by ChIP-chip analysis. We also identified androgen responsive miRNAs and ncRNAs by large scale screening such as CAGE or short RNA sequence. Notably, we showed miR-148a is a primary androgen regulated miRNA strongly expressed in prostate cancer cells. Furthermore, we also found MiR-148a promotes prostate cancer cell proliferation.

Research Products

• [Journal Article] Integration of cap analysis of gene expression and chromatin immunoprecipitation analysis on array reveals genome-wide androgen receptor signaling in prostate cancer cells. (2011)
  • Author(s): Takayama K, Tsutsumi S, Katayama S, Okayama T, Horie-Inoue K, Ikeda K, Urano T, Kawazu C, Hasegawa A, Ikeo K, Gojyobori T, Ouchi Y, Hayashizaki Y, Aburatani H, Inoue S
  • Journal Title: Oncogene 30 Pages: 619-630
  • Peer Reviewed
• [Journal Article] Differential expression of estrogen-related receptors β and γ (ERRβ and ERRγ) and their clinical significance in human prostate cancer. (2010)
  • Author(s): Fujimura T, Takahashi S, Urano T, Ijichi N, Ikeda K, Kumagai J, Murata T, Takayama K, Horie-Inoue K, Ouchi Y, Muramatsu M, Homma Y, Inoue S
  • Journal Title: Cancer Sci 101 Pages: 646-651
  • Peer Reviewed
• [Journal Article] miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression. (2010)
  • Author(s): Murata T, Takayama K, Katayama S, Urano T, Horie-Inoue K, Ikeda K, Takahashi S, Kawazu C, Hasegawa A, Ouchi Y, Homma Y, Hayashizaki Y, Inoue S
  • Journal Title: Prostate Cancer Prostatic Dis 13 Pages: 356-361
  • Peer Reviewed
• [Journal Article] Oct1 regulates cell growth of LNCaP cells and is a prognostic factor for prostate cancer.
  • Author(s): Obinata D, Takayama K, Urano T, Murata T, Kumagai J, Fujimura T, Ikeda K, Horie-Inoue K, Homma Y, Ouchi Y, Takahashi S, Inoue S
  • Journal Title: Int J Cancer in press
  • Peer Reviewed
• [Journal Article] ARFGAP3, an androgen target gene, promotes prostate cancer cell proliferation and migration.
  • Author(s): Obinata D, Takayama K, Urano T, Murata T, Ikeda K, Horie-Inoue K, Ouchi Y, Takahashi S, Inoue S
  • Journal Title: Int J Cancer in press
  • Peer Reviewed
• [Presentation] Integrative analysis of androgen receptor signaling in prostate cancer cells by cap analysis of gene expression (CAGE) and chromatin immunoprecipitation analysis on array in prostate cancer cells. (2010)
  • Author(s): Takayama K, Tsutsumi S, Katayama S, Horie-Inoue K, Ikeda K, Urano T, Ouchi Y, Hayashizaki Y, Aburatani H, Inoue S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会 BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] ChIP analysis on array reveals genome-wide androgen receptor-binding sites in human prostate cancer cells. (2010)
  • Author(s): Takayama K , Tsutsumi S, Horie-Inoue K, Ikeda K, Urano T, Ouchi Y, Aburatani H, Inoue S
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-20100924
• [Presentation] [学会賞(基礎部門)受賞] 次世代シーケンサーを活用したアンドロゲン依存性転写開始点の包括的同定による前立腺癌におけるアンドロゲン標的シグナルの解析 (2010)
  • Author(s): 高山賢一、片山慎太郎、堀江公仁子、池田和博、浦野友彦、大内尉義、林崎良英、井上聡
  • Organizer: 第29回日本アンドロロジー学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 20100730-20100731
• [Presentation] ヒト21、22番染色体におけるアンドロゲン受容体結合部位の探索 (2010)
  • Author(s): 高山賢一、浦野友彦、大内尉義、井上聡
  • Organizer: 第52回日本老年医学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20100624-20100626
• [Presentation] A cell cycle regulator, transforming acidic coiled-coil protein 2 (TACC2), is an androgen-regulated gene that promotes prostate cancer progression. (2010)
  • Author(s): Takayama K, Suzuki T, Horie-Inoue K, Ikeda K, Urano T, Ouchi Y, Inoue S
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 20100326-20100330
• [Presentation] Global analysis of Androgen receptor binding sites in human prostate cancer cells. (2010)
  • Author(s): Takayama K, Tsutsumi S, Horie-Inoue K, Ikeda K, Urano T, Ouchi Y, Aburatani H, Inoue S
  • Organizer: Satellite Symposia, Nuclear Receptor and its Frontier, 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2010-03-31
• [Presentation] RUNX1 is a novel androgen- regulated gene that modulates AR transcriptional activity and prostate cancer cell proliferation. (2009)
  • Author(s): Takayama K, Tsutsumi S, Horie-Inoue K, Ikeda K, Urano T, Ouchi Y, Aburatani H, Inoue S
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20091001-20091003
• [Presentation] [YIA受賞]ヒトゲノムにおけるアンドロゲン受容体結合部位ならびにヒストン蛋白修飾の包括的解析による新規前立腺癌診断・治療標的の同定 (2009)
  • Author(s): 高山賢一、堀江公仁子、池田和博、浦野友彦、鈴木貴、藤村哲也、高橋悟、堤修一、油谷浩幸、大内尉義、井上聡
  • Organizer: 第82回日本内分泌学会学術集会
  • Place of Presentation: 群馬
  • Year and Date: 20090423-20090425
• [Remarks] 特になし