2011 Fiscal Year Final Research Report
Functional analysis of CD133in tumorigenesis and stemness of cancer stem cell
| Project/Area Number |
21790397
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Chiba Cancer Center (Research Institute) |
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Principal Investigator |
SHIMOZATO Osamu 千葉県がんセンター(研究所), 発がん研究グループ, 上席研究員 (30344063)
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| Project Period (FY) |
2009 – 2011
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| Research Abstract |
CD133is a putative cancer stem cell marker of several malignancies but its roles still remain unclear. We here examined roles of CD133in tumorigenesis and stemness of human colon cancer cells. Knocked-down of CD133retarded tumor formation of colon cancer cells in nude mice and suppressed anchorage-independent cell growth. CD133-knockdown reduced transactivation activity ofβ-catenin resulting from AKT inactivation ; subsequently accelerated the enterocyte differentiation of colon cancer cells upon sodium butyrate treatment. Furthermore, we found a novel transcriptional regulation of CD133gene by GATA6transcription factor, which is bound to the upstream region of exon2of CD133gene. These data collectively suggest that CD133is involved in keeping undifferentiated-status and tumorigenesis of colon cancer.
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