2010 Fiscal Year Final Research Report
Apicomplexa specific traffic pathway in protozoa
| Project/Area Number |
21790411
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
NAKANO Yumiko (斉藤 由美子) National Institute of Infectious Diseases, 寄生動物部, 主任研究官 (30321764)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 原虫 |
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| Research Abstract |
One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.
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Research Products
(4 results)
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[Journal Article] Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia.2010
Author(s)
Saito-Nakano.Y., Nakahara,T., Nakano,K., Nozaki,T .,Numata,O
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Journal Title
The Journal of Eukaryotic Microbiology. 57
Pages: 389-399
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[Presentation] Dive rsity and Significance of Vesicular Trafficking in Entamoeba histolytica.2010
Author(s)
Yumiko Saito-Nakano, Mami Okada,Gil Mallari Penuliar, Yuki Hanadate, Carol, A., Gilchrist, Oswald Crasta, Wil liam, A., Petri,Jr., Zhangjun Fei, Nino Trapaidze, Tomoyoshi Nozaki
Place of Presentation
Holel le Crystal(カナダ国)
Year and Date
20100922-20100924
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