Analysis of pathogen recognition mechanism in autophagy, and analysis of bacterial evasion mechanism from autophagic recognition.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790413
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: The University of Tokyo
• Principal Investigator: OGAWA Michinaga The University of Tokyo, 医科学研究所, 助教 (80361624)
• Project Period (FY): 2009 – 2010
• Keywords: 病原性 / 赤痢菌

Research Abstract

We found that Listeria monocytogenes ActA, a surface protein required for actin polymerization and actin-based bacterial motility, plays a pivotal role in evading autophagy, but in a manner independent of bacterial motility. L. monocytogenes expressing ActA mutants that lack the ability to recruit the host proteins initially underwent ubiquitylation, followed by recruitment of p62 and LC3, before finally undergoing autophagy. We found that the involvement of Afp, newly discovered Atg5-interacting protein, in autophagy targeting Shigella.

Research Products

• [Journal Article] Autophagy targeting of Listeria monocytogenes and the bacterial countermeasure. (2011)
  • Author(s): Ogawa, M., Yoshikawa, Y., Mimuro, H., Hain, T., Chakraborty, T., Sasakawa, C.
  • Journal Title: Autophagy 7
• [Journal Article] Shigella deploy multiple countermeasures against host innate immune responses. (2011)
  • Author(s): Ashida, H., Ogawa, M., Kim, M., Suzuki, S., Sanada, T., Punginelli, C., Mimuro, H., Sasakawa, C.
  • Journal Title: Curr Opin Microbiol 14 Pages: 16-23
• [Journal Article] Reinforcement of epithelial cell adhesion to basement membrane by a bacterial pathogen as a new infectious stratagem. (2010)
  • Author(s): Kim, M., Ogawa, M., Mimuro, H., Sasakawa, C.
  • Journal Title: Virulence 1 Pages: 52-55
• [Journal Article] Bacterial interactions with the host epithelium. (2010)
  • Author(s): Kim, M., Ashida, H., Ogawa, M., Yoshikawa, Y., Mimuro, H., Sasakawa, C.
  • Journal Title: Cell Host Microbe 8 Pages: 20-35
• [Journal Article] A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. (2010)
  • Author(s): Ashida, H., Kim, M., Schmidt-Supprian, M., Ma, A., Ogawa, M., Sasakawa, C.
  • Journal Title: Nat Cell Biol 12 Pages: 66-73,61-69
• [Journal Article] Shigella strategy for host defense and mucosal infection (2009)
  • Author(s): 小川道永、笹川千尋
  • Journal Title: 蛋白質核酸酵素 54 Pages: 988-995
• [Journal Article] 細菌感染とオートファジー (2009)
  • Author(s): 小川道永、吉川悠子、笹川千尋
  • Journal Title: 臨床検査 53 Pages: 1569-1575
• [Journal Article] Listeria monocytogenes ActA-mediated escape from autophagic recognition. (2009)
  • Author(s): Yoshikawa, Y., Ogawa, M., Hain, T., Yoshida, M., Fukumatsu, M., Kim, M., Mimuro, H., Nakagawa, I., Yanagawa, T., Ishii, T., Kakizuka, A., Sztul, E., Chakraborty, T., Sasakawa, C.
  • Journal Title: Nat Cell Biol 11 Pages: 1233-1240
• [Journal Article] Listeria monocytogenes ActA is a key player in evading autophagic recognition. (2009)
  • Author(s): Yoshikawa, Y., Ogawa, M., Hain, T., Chakraborty, T., Sasakawa, C.
  • Journal Title: Autophagy 5 Pages: 1220-1221
• [Journal Article] Streptococcus-, Shigella-, and Listeria-induced autophagy. (2009)
  • Author(s): Ogawa, M., Nakagawa, I., Yoshikawa, Y., Hain, T., Chakraborty, T., Sasakawa, C.
  • Journal Title: Methods Enzymol 452 Pages: 363-381
• [Journal Article] Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. (2009)
  • Author(s): Kim, M., Ogawa, M., Fujita, Y., Yoshikawa, Y., Nagai, T., Koyama, T., Nagai, S., Lange, A., Fassler, R., Sasakawa, C.
  • Journal Title: Nature
• [Journal Article] Shigella infection of intestinal epithelium and circumvention of the host innate defense system. (2009)
  • Author(s): Ashida, H., Ogawa, M., Mimuro, H., Sasakawa, C.
  • Journal Title: Curr Top Microbiol Immunol 337 Pages: 231-255
• [Remarks] 吉川悠子ホームページ等