2010 Fiscal Year Final Research Report
Exploring the factors of Mycobacterium tuberculosis that function in the dissociation of Rab GTPases from mycobacterial phagosomes
| Project/Area Number |
21790415
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
SETO Shintaro Hamamatsu University School of Medicine, 医学部, 助教 (50383203)
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| Project Period (FY) |
2009 – 2010
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| Research Abstract |
As the candidate of mycobacterial factor that function in the dissociation of Rab GTPases from mycobacterial phagosomes, we focused on the gene Rv3377c. It is reported that Rv3377c product has the cyclase activity for geranylgeranyl moieties. We found that the attenuated strains Mycobacterium tuberculosis H37Ra and Mycobacterium bovis BCG do not express Rv3377c in infected macrophages. Next, we explored the host factors that support the proliferation of M. tuberculosis in the phagosomes in infected macrophages. we carried out the proteomic analysis of mycobacterial phagosomes isolated from infected macrophages. Raw264.7 macrophages were infected with M.tuberculosis, and phagosomal fraction was isolated by sucrose gradient centrifugation. Proteins extracted from mycobacterial phagosomes were subjected to two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectromety. The prominent proteins in the fraction of mycobacterial phagosome were those associated with the endoplasmic reticulum (ER), suggesting that the vesicles derived from ER interact with mycobacterial phagosomes during the inhibition of phagosome maturation.
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