2011 Fiscal Year Final Research Report

Inhibitory effect of NaCl on the production of extracellular proteins by Aeromonas spp.

Research Project

Project/Area Number	21790422
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Bacteriology (including Mycology)
Research Institution	Okayama University
Principal Investigator	TAKAHASHI Eizo 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70379733)
Project Period (FY)	2009 – 2011
Keywords	病原性 / 病原毒素 / アエロモナス(エロモナス) / プロテアーゼ / リパーゼ / NaCl
Research Abstract	Aeromonas, which is a causative agent of diarrhea of adults and children, could not produce active metalloprotease and lipase in the medium containing 3% NaCl, though it could produce them under physiological condition. In this study, we constructed the mutant strain in which the gene encoding LysR type transcriptional factor is deleted and examined whether the deletion of LysR affects the production of metalloprotease. The results show that LysR type transcriptional factor is not involved in production of metalloprotease. Additionally, we found the lipase gene is transcribed in the medium containing 3% NaCl as well as in the medium 0. 5% NaCl, though production of lipase is reduced under salty condition. And we showed that the lipase produced slightly in the medium containing 3% NaCl is denatured.