Differential regulation of master regulatory switch PchABC for the type 3 secretion system in Escherichia coli.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790434
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: IYODA Sunao National Institute of Infectious Diseases, 細菌第一部, 主任研究官 (70300928)
• Research Collaborator: 本田 尚子 東京大学, 大学院・医学系研究科, 博士後期課程大学院生
• Project Period (FY): 2009 – 2010
• Keywords: 腸管出血性大腸菌 / 発現制御

Research Abstract

The transcription of LEE genes in EHEC is under the positive control of PchABC, which are encoded by the genes dispersed on the chromosome. We identified transcriptional start sites and promoter sequences of each gene. Genetic analysis using transposon mutagenesis identified that a LysR-type regulator, LrhA positively controls LEE gene expression through the transcriptional up-regulation of pchA and pchB.

Research Products

• [Journal Article] New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups : 0157, 026, and 0111. (2010)
  • Author(s): Izumiya H, Pei Y, Terajima J, Ohnishi M, Hayashi T, Iyoda S, Watanabe H
  • Journal Title: Microbiol Immunol. 54 Pages: 569-577
  • Peer Reviewed
• [Journal Article] Complete genome sequence of the wild-type commensal Escherichia coli strain SE15, belonging to phylogenetic group B2. (2010)
  • Author(s): Toh H, Oshima K, Toyoda A, Ogura Y, Ooka T, Sasamoto H, Park SH, Iyoda S, Kurokawa K, Morita H, Itoh K, Taylor TD, Hayashi T, Hattori M
  • Journal Title: J Bacteriol. 192 Pages: 1165-1166
  • Peer Reviewed
• [Journal Article] LrhA positively controls the expression of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coli by differential regulation of their master regulators PchA and PchB. (2009)
  • Author(s): Honda N, Iyoda S, Yamamoto S,Terajima J, Watanabe H
  • Journal Title: Mol. Microbiol. 74 Pages: 1393-1411
  • Peer Reviewed
• [Journal Article] Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli 0157 strains. (2009)
  • Author(s): Ooka T, Terajima J, Kusumoto M, Iguchi A, Kurokawa K, Ogura Y, Asadulghani M, Nakayama K, Murase K, Ohnishi M, Iyoda S, Watanabe H, Hayashi T
  • Journal Title: J.Clin. Microbiol. 47 Pages: 2888-2894
  • Peer Reviewed
• [Journal Article] The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA. (2009)
  • Author(s): Shimuta K, Ohnishi M, Iyoda S, Gotoh N, Koizumi N, Watanabe H
  • Journal Title: BMC Microbiol.on-line jouornal 9
  • Peer Reviewed
• [Presentation] 腸管出血性大腸菌におけるLEE遺伝子群のマスターレギュレーターPchのグローバル発現制御機構の解析 (2010)
  • Author(s): 伊豫田淳,寺嶋淳,大西真
  • Organizer: 第93回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-21
• [Presentation] LEE遺伝子群のマスターレギュレーターPchの分別発現制御機構の解析 (2010)
  • Author(s): 伊豫田淳、本田尚子、寺嶋淳、大西真
  • Organizer: 第14回腸管出血性大腸菌感染症シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2010-07-23
• [Presentation] 腸管出血性大腸菌ワーキンググループVirulence traits of EHEC 0157:H7 clade 8, a possible high virulent lineage. (2010)
  • Author(s): 伊豫田淳、寺嶋淳、泉谷秀昌、大西真、渡邉治雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] クレード解析によるDNA型別から明らかとなった高病原性と推定される腸管出血性大腸菌0157:H7株の解析 (2009)
  • Author(s): 伊豫田淳、寺嶋淳、泉谷秀昌、大西真、渡邉治雄
  • Organizer: 第92回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-05
• [Presentation] クレード解析による腸管出血性大腸菌のDNA型別 (2009)
  • Author(s): 伊豫田淳、寺嶋淳、泉谷秀昌、大西真、渡邉治雄
  • Organizer: 第13回腸管出血性大腸菌感染症シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-16
• [Presentation] 日本国内における大腸菌0157:H7食中毒発生状況と分離株の性状 (2009)
  • Author(s): 伊豫田淳, 寺嶋淳, 泉谷秀昌, 渡邉治雄
  • Organizer: 第147回日本獣医学会学術集会・ワークショップ
  • Place of Presentation: 栃木
  • Year and Date: 2009-04-04