2010 Fiscal Year Final Research Report
Analysis of dengue virus quasispecies and their cell tropism.
| Project/Area Number |
21790444
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
KUROSU Takeshi Osaka University, 微生物病研究所, 助教 (70432432)
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| Research Collaborator |
ANANTAPREECHA Surapee National Institute of Health, Department of Medical Sciences, Thailand
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| Project Period (FY) |
2009 – 2010
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| Keywords | デングウイルス / フラビウイルス / ウイルス疑似種 / 感染症 / 熱帯病 |
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| Research Abstract |
Dengue virus (DENV) is known to exist as various virus population, so-called quasispecies. In this study, we focused on the DENV quasispecies to take a new approach to analyze DENV. DENV easily cause severe illness when patients are secondary infected with DENV. We analyzed the sequences of DENV derived from primary and secondary infection, and then found that the DENV from primary infections carried broad range of variation in DENV genomes, contrary DENV from secondary infection were homogenous. This suggests that DENV from secondary infection effectively selected in secondary infections. Furthermore, we developed an effective protocol for the analysis of DENV quasispecies and observed the virus population was switch to one population.
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