2010 Fiscal Year Final Research Report
Analysis of the uptake mechanism for double stranded RNA
| Project/Area Number |
21790457
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Hokkaido University |
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Principal Investigator |
WATANABE Ayako Hokkaido University, 大学院・医学研究科, 博士研究員 (00533423)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 二重鎖RNA / 膜受容体 / Toll-like receptor 3 |
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| Research Abstract |
we show that the cytoplasmic lipid raft protein, Raftlin, is essential for poly(I:C) cellular uptake in human myeloid DCs and epithelial cells. When Raftlin was silenced, poly(I:C) failed to enter cells and induction of IFN-beta production was inhibited. In addition, cellular uptake of B-type oligodeoxynucleotide that shares its uptake receptor with poly(I:C) was suppressed in Raftlin knockdown cells. Upon poly(I:C) stimulation, Raftlin was translocated from the cytoplasm to the plasma membrane where it colocalized with poly(I:C), and thereafter moved to TLR3-positive endosomes. Thus, Raftlin cooperates with the uptake receptor to mediate cell entry of poly(I:C), which is critical for activation of TLR3.
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