2010 Fiscal Year Final Research Report
Novel analysis method of hematopoiesis applying to embryonic lethal mice
| Project/Area Number |
21790480
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
WADA Haruka St.Marianna University School of Medicine, 医学(系)研究科(研究院), 講師 (70392181)
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| Project Period (FY) |
2009 – 2010
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| Keywords | iPS細胞 / T細胞 / B細胞 / 造血前駆細胞 / 分化誘導 / リプログラミング |
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| Research Abstract |
Forced expression of certain transcription factors in somatic cells results in generation of induced pluripotent stem (iPS) cells, which differentiate into various cell types. We investigated T-cell and B-cell lineage differentiation from iPS cells in vitro. iPS cells differentiated into lymphocytes in OP9 co-culture systems. iPS cells efficiently differentiated into T-cell lineage. However, iPS cells including B-iPS cells were relatively resistant to B-cell lineage differentiation. One of the reasons of the failure of B-cell lineage differentiation seemed due to a defect of Pax5 expression in the differentiated cells. Therefore, current in vitro differentiation systems using iPS cells are sufficient for inducing T-cell but not B-cell lineage.
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[Journal Article] Establishment of an improved mouse model for infantile neuroaxonal dystrophy that shows early disease onset and bears a point mutation in Pla2g62009
Author(s)
Wada H, Yasuda T, Miura I, Watabe K, Sawa C, Kamijuku H, Kojo S, Taniguchi M, Nishino I, Wakana S, Yoshida H, Seino K.
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Journal Title
Am J Pathol. 175
Pages: 2257-2263
Peer Reviewed
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