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2010 Fiscal Year Final Research Report

Visualization and molecular analysis of antigen-uptake and -delivery at M cell.

Research Project

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Project/Area Number 21790487
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KIMURA Shunsuke  The Institute of Physical and Chemical Research, 大学院・医学研究科, 助教 (40444525)

Project Period (FY) 2009 – 2010
Keywordsパイエル板 / M細胞
Research Abstract

Peyer's patch (PP) in the small intestine is a primary site for the intestinal immune system. The luminal side of PPs is covered by the follicle-associated epithelium (FAE), which contains M cells. M-cells uptake antigens or macromolecules and deliver them to dendritic cells inhabiting subepithelial dome region under FAE. We found high expression level of a chemokine gene, Ccl9, in the M cells by using the mouse chemokine array and in situ hybridization analysis. The cells expressing CCR1 protein, a receptor of CCL9, were found underneath M cells and close contact with. Moreover, M cells expressed a gene of M-Sec which product is a key molecule of the formation of membrane nanotubes, a new structure for cell-cell communication. Taken together, our data suggest that M cells interact and communicate with immune cells by two ways, CCL9-CCR1 and membrane nanotubes.

  • Research Products

    (9 results)

All 2011 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice.2011

    • Author(s)
      Takahashi D, Hase K, Kimura S, Nakatsu F, Ohmae M, Mandai Y, Sato T, Date Y, Ebisawa M, Kato T, Obata Y, Fukuda S, Kawamura Y, Dohi D, Katsuno T, Yokosuka O, Waguri S, Ohno H
    • Journal Title

      Gastroenterology

    • Peer Reviewed
  • [Journal Article] M-Sec : Emerging secrets of tunneling nanotube formation.2010

    • Author(s)
      Ohno H, Hase K, Kimura S
    • Journal Title

      Commun Integr Biol. 3(3)

      Pages: 231-233

    • Peer Reviewed
  • [Journal Article] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.2009

    • Author(s)
      Hase K, Kimura S, Takatsu H, Ohmae M, Kawano S, Kitamura H, Ito M, Watarai H, Hazelett CC, Yeaman C, Ohno H.
    • Journal Title

      Nat Cell Biol 11(12)

      Pages: 1427-1432

    • Peer Reviewed
  • [Journal Article] Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response.2009

    • Author(s)
      Hase K, Kawano K, Nochi T, Pontes GS, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe AW, Itoh K, Kiyono H, Ohno H.
    • Journal Title

      Nature 462(7270)

      Pages: 226-230

    • Peer Reviewed
  • [Journal Article] Monitoring autophagy in mammalian cultured cells through the dynamics of LC3.2009

    • Author(s)
      Kimura S, Fujita N, Noda T, Yoshimori T
    • Journal Title

      Methods Enzymol 452

      Pages: 1-12

    • Peer Reviewed
  • [Presentation] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex2010

    • Author(s)
      Koji Hase, Shunsuke Kimura, Hiroshi Ohno
    • Organizer
      第14回国際免疫学会議
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-08-26
  • [Presentation] Functional analysis of CCL9 in small intestine2009

    • Author(s)
      Ryo Yamazaki, Shunsuke Kimura, Masashi Ebisawa, Yasuhiro Date, Satoshi Tsuneda, Haruhiko Suzuki, Koji Hase, Hiroshi Ohno
    • Organizer
      日本免疫学会
    • Place of Presentation
      大阪府大阪市
    • Year and Date
      2009-12-02
  • [Presentation]2009

    • Author(s)
      Shunsuke Kimura, Ayako Sakamoto, Ryo Yamazaki, Kazuya Kawano, Koji Hase, Hiroshi Ohno
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2009-06-02
  • [Presentation] Functional analysis of CCL9 in small intestine2009

    • Author(s)
      Ryo Yamazaki, Shunsuke Kimura, Koji Hase, Masashi Ebisawa, Yasuhiro Date, Hiroshi Ohno
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2009-06-02

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Published: 2012-02-13   Modified: 2016-04-21  

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