2010 Fiscal Year Final Research Report
Establishment of the effective cell culture method for regulatory T cells.
| Project/Area Number |
21790535
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
SHIBAKURA Misako Okayama University, 大学院・保健学研究科, 准教授 (30314694)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 制御性T細胞 / 培養方法 / P-セレクチン / PSGL-1 |
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| Research Abstract |
I tried the establishment of the effective cell culture method of regulatory T cells. To clarify the role of selectins/selectin-ligands in the cell proliferation and the function of Treg cells, we examined the cell proliferation rate and the function of Treg cells, using P-selectin-/-mice and PSGL-1-/-mice. The growth rate of P-selectin-/-Treg cells and PSGL-1-/-Treg cells were high in comparison with the wild-type Treg cells. PSGL-1-/-Treg cells could inhibit wild-type T cell proliferation in in-vitro suppression assay.
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