2010 Fiscal Year Final Research Report

Development of "microRNA-regulating chemoprevention"

Research Project

Project/Area Number	21790555
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Hygiene
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	TOMOSUGI Mitsuhiro Kyoto Prefectural University of Medicine, 医学部, プロジェクト研究員 (60533429)
Project Period (FY)	2009 – 2010
Keywords	癌 / microRNA / 予防法開発 / 細胞周期停止 / RB
Research Abstract	miR-34a is a tumor suppressor microRNA. I searched for the food components that regulated miR-34a expression. As a result from flow cytometry analysis, several kinds of materials inducing G1 arrest were found, using RB inactivated cancer cells. Real-time PCR specified flavone that induced miR-34a expression from candidate materials. In addition, decrease in target proteins of miR-34a was confirmed by Western blot analysis. These results suggested that food component could control expression of microRNA in vivo. The research of miR-34a regulated by flavone might realize "microRNA-regulating chemoprevention"

(3 results)