2010 Fiscal Year Final Research Report

The absorption and metabolism of arsenic species in intestinal and tubular epithelial cells.

Research Project

Project/Area Number	21790558
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Hygiene
Research Institution	Chiba Institute of Science
Principal Investigator	HATA Akihisa Chiba Institute of Science, 危機管理学部, 助手 (10433690)
Project Period (FY)	2009 – 2010
Keywords	arsenic / speciation / biological monitoring / seafood / occupational exposure / urine / renal tubule
Research Abstract	MDCK cells and Caco-2 cells did not alter the chemical form of arsenate, arsenite, monomethylarsonic acid, dimethylarsinic acid, trimethylarsine oxide, arsenocholine, and arsenobetaine. The permeability of these 7 arsenic species was studied in a renal tubular epithelial model comprising MDCK monolayer, and was found to be 1-2.5-times higher than that of creatinine. The arsenic compounds in fish and seaweeds were effectively extracted using 50% methanol (extractant) and bead-beating tube (physical extraction method).

(3 results)