2010 Fiscal Year Final Research Report
Development of a quantitative assay for the allergen of oral allergy syndrome
| Project/Area Number |
21790602
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
KIYOTA Kyohei Osaka Prefectural Institute of Public Health, 衛生化学部, 研究員 (10516743)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 口腔アレルギー症候群 / アレルゲン / 抗体 / ELISA |
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| Research Abstract |
In this study, I have produced three types of antibodies to recognize profilin, which is one of the major allergens of oral allergy syndrome, and have tried to develop a quantitative ELISA to measure profilin using these antibodies. As a result, in direct ELISA, the linearity of the standard curve was good and it was possible to quantify profilin concentrations in cucumber and tomato. On the other hand, in sandwich ELISA, the detection sensitivity was not enough. Thus, it is necessary to develop a high sensitive ELISA in the next study. The information of allergen content in food is important for selecting foods by consumers and would be useful in preventing the onset of oral allergy syndrome.
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