Molecular mechanisms of ethanol dependence and relapse-role in the brain microRNA

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790611
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Sapporo Medical University
• Principal Investigator: MIZUO Keisuke Sapporo Medical University, 医学部, 助教 (90459735)
• Project Period (FY): 2009 – 2010
• Keywords: アルコール / マイクロRNA / 依存

Research Abstract

Prolonged ethanol intake leads to the development of ethanol dependence. Although a lot of study suggested several candidates, certain mechanism of ethanol action is still unclear. Here we show that acute ethanol administration increased the expression of microRNA in mouse brain at 12 hours after administration. We observed that the acute ethanol administration significantly increased the levels of acetylated histone H3 in nucleus accumbens, ventral tegmental area and amygdala. The expression of miR-124 was significantly increased in limbic forebrain and lower midbrain following chronic treatment of ethanol. These findings suggest that chronic ethanol consumption increase the microRNA expression via histone acetylation, resulting in development of ethanol dependence.

Research Products

• [Journal Article] Effect of acute ethanol administration on histone deacetylases in mouse brain. (2011)
  • Author(s): Mizuo.K., Katada, R., Tateda, K., Okazaki, S., Watanabe, S., Matsumoto, T.
  • Journal Title: Alcohol Biomed.Res. In press
• [Journal Article] Expression of miR-132 in murine brain after acute ethanol administration. (2010)
  • Author(s): Mizuo.K., Nishitani, Y., Katada, R., Tateda, K., Okazaki, S., Watanabe, S., Matsumoto, T.
  • Journal Title: Alcohol Biomed.Res. 29 Pages: 52-54
• [Presentation] アルコールの急性投与は脳におけるmicroRNA発現に影響を及ぼすか (2010)
  • Author(s): 水尾圭祐
  • Organizer: 包括脳ネットワーク
  • Place of Presentation: ホテルさっぽろ芸文館(札幌市)
  • Year and Date: 20100728-20100729
• [Presentation] エタノール急性投与が脳におけるヒストン脱アセチル化酵素に及ぼす影響 (2010)
  • Author(s): 水尾圭祐
  • Organizer: アルコール医学生物学研究会
  • Place of Presentation: 久留米翠香園ホテル(久留米市)
  • Year and Date: 2010-11-26
• [Presentation] エタノール急性投与は脳におけるヒストンアセチル化を促進する (2010)
  • Author(s): 水尾圭祐
  • Organizer: 日本アルコール・薬物医学会
  • Place of Presentation: リーガロイヤルホテル小倉(小倉市)
  • Year and Date: 2010-10-09
• [Presentation] Acute ethanol admi nistration changes in the expression of microRNAs in mouse brain. (2010)
  • Author(s): Keisuke Mizuo
  • Organizer: International Society for Biomedical Research on Alcoholism
  • Place of Presentation: CAPS15(パリ市)
  • Year and Date: 2010-09-16
• [Presentation] エタノール急性投与が脳におけるヒストンアセチル化に及ぼす影響 (2010)
  • Author(s): 水尾圭祐
  • Organizer: 日本神経科学学会
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
  • Year and Date: 2010-09-04
• [Presentation] エタノールの急性投与は脳におけるmiR-124の発現を増加させる (2010)
  • Author(s): 水尾圭祐
  • Organizer: 日本法医学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2010-06-24
• [Presentation] アルコールによる中枢神経系におけるmiR-132の発現動態 (2009)
  • Author(s): 水尾圭祐
  • Organizer: アルコール医学生物学研究会
  • Place of Presentation: 三井ガーデンホテル千葉(千葉市)
  • Year and Date: 2009-11-13
• [Presentation] エタノール急性投与による脳におけるマイクロRNAの発現変動 (2009)
  • Author(s): 水尾圭祐
  • Organizer: 日本アルコール・薬物医学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-09-09