2010 Fiscal Year Final Research Report
The genetic change of pancreatic duct epithelial cells mediated by epithelial mesenchymal transition
| Project/Area Number |
21790645
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
KANNO Atsushi Tohoku University, 病院, 助教 (70509190)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 膵癌 / 慢性膵炎 / K-ras / miRNA / 膵星細胞 |
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| Research Abstract |
1. The establish of the Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation
We established that Human Pancraetic Duct Epithelial Cell (HPDE) which retroviral vector pBabepuro-KRAS4B^<G12V> was transfected. We confirmed that the HPDE had K-ras mutation. We are trying that retroviral vector pBabepuro-KRAS4B^<G12V> was transfected to the human pancreatic stellate cell and the rat pancreatic stellate cell in the similar medhod.
2. The alteration of the gene by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell.
(1) We are researching that the alteration of the gene by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell.
(2) We are examining that the alteration of the miRNA by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell with miRNA microarray.
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Research Products
(16 results)
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[Journal Article] Granular cell tumor of the pancreas : A case report and review of literature.2010
Author(s)
Kanno A, Satoh K, Hirota M, Hamada S, Umino J, Itoh H, Masamune A, Egawa S, Motoi F, Unno M, Ishida K, Shimosegawa T.
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Journal Title
World J Gastrointest Oncol. 2(2)
Pages: 121-124
Peer Reviewed
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[Presentation] Standard steroid therapy for autoimmune pancreatitis2010
Author(s)
A Kanno, T Kamisawa, T Shimosegawa, K Okazaki, T Nishino, H Watanabe, F Okumura, T Nishikawa, K Kobayashi, T Ichiya, H Takatori, K Yamakita, K Kubota, H Hamano, K Okamura, K Hirano, T Ito, S B H Ko, M Omata
Organizer
IAP and JPS 2010
Place of Presentation
Fukuoka
Year and Date
2010-07-10
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[Presentation] 下部胆管に発生した腺内分泌細胞癌の一例2009
Author(s)
菅野敦, 佐藤賢一, 正宗淳, 廣田衛久, 菊田和弘, 粂潔, 濱田晋, 渡辺崇, 伊藤広通, 下瀬川徹, 高舘達之, 力山敏樹, 海野倫明, 石田和之
Organizer
第45回日本胆道学会
Place of Presentation
千葉
Year and Date
2009-09-18
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