2010 Fiscal Year Final Research Report
Analyses of cell-cell interaction regulating proliferation and differentiation of hepatic stem cells during early-liver development
| Project/Area Number |
21790650
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAMIYA Akihide The University of Tokyo, 医科学研究所, 助教 (30321904)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 肝幹細胞 / 肝再生 / 肝臓病学 |
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| Research Abstract |
Hepatic stem/progenitor cells (HSPCs) in the liver bud are postulated to migrate into septum transversum mesenchyme at around embryonic day (E) 9 in mice. Mid-fetal livers (E11.5-E14.5) support proliferation and differentiation of hematopoietic stem cells derived from the aorta-gonad-mesonephros region. We established a new co-culture system, using mouse embryonic fibroblast cells, to permit analysis of the phenotypes of early-fetal HSPC candidates. In this co-culture system, CD13^+Dlk^+ cells purified from mouse early-fetal livers (E9.5 and E10.5) formed colonies composed of both albumin-positive hepatocytic cells and cytokeratin (CK) 19-positive cholangiocytic cells, indicating that early-fetal CD13^+Dlk^+ cells have properties of bi-potent progenitor cells. These first prospective studies of early-fetal HSPC candidates demonstrate that bi-potent stem/progenitor cells exist before E11.5.
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[Journal Article] Bmi1 promotes hepatic stem cell expansion and tumorigenicity in both Ink4a/Arf-dependent and independent manners.2010
Author(s)
Chiba T, Seki A, Aoki R, Ichikawa H, Negishi M, Miyagi S, Oguro H, Saraya A, Kamiya A, Nakauchi H, Yokosuka O, Iwama A.
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Journal Title
Hepatology 52
Pages: 1111-1123
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[Remarks] ホームページ等
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