The effect of human neutrophil peptides on intestinal inflammation as determined by proteomics approaches

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790672
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Kagoshima University
• Principal Investigator: KANMURA Shuji Kagoshima University, 医学部・歯学部附属病院, 医員 (60448561)
• Project Period (FY): 2009 – 2010
• Keywords: ディフェンシン / 炎症性腸疾患 / 腸管免疫 / 抗菌ペプチド / プロテオミクス / 潰瘍性大腸炎 / サイトカイン / 好中球

Research Abstract

Human neutrophil peptides (HNPs) 1-3 promoted proliferation of epithelial cell lines at low peptide concentrations ; however, higher peptide concentrations inhibited cellular proliferation. And, treatment with HNPs resulted in the increase expression of interleukin-1 (IL-8), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) from epithelial cell lines. Additionally, after administration of HNPs to DSS-induced mouse IBD model, high histological colitis score, weight loss, and shortening of the large intestine were observed as compared to non-treated mice. These results suggest that HNPs increase the expression of proinflammatory cytokines in intestinal epithelial cells and exacerbates experimental colitis.

Research Products

• [Journal Article] プロテオーム解析にもとづくIBDの診断 (2010)
  • Author(s): 上村修司、宇都浩文、嵜山敏男、坪内博仁
  • Journal Title: IBDresearch 4 Pages: 288-293
• [Journal Article] Clinical proteomics for liver disease : a promising approach for discovery of novel biomarkers. (2010)
  • Author(s): Uto H, Kanmura S, Takami Y, Tsubouchi H.
  • Journal Title: Proteome Sci. 31 Pages: 70
  • Peer Reviewed
• [Journal Article] The complement component C3a fragment is a potential biomarker for hepatitis C virus-related hepatocellular carcinoma.J Gastroenterol. (2010)
  • Author(s): Kanmura S, Uto H, Sato Y, Kumagai K, Sasaki F, Moriuchi A, Oketani M, Ido A, Nagata K, Hayashi K, Stuver SO, Tsubouchi H.
  • Journal Title: J Gastroenterol. 45 Pages: 459-467
  • Peer Reviewed
• [Journal Article] 炎症性腸疾患の新規治療を探る「プロテオーム解析からの治療戦略とは?」 (2009)
  • Author(s): 上村修司、宇都浩文、坪内博仁
  • Journal Title: 分子消化器病 6 Pages: 123-127
• [Journal Article] Human Neutrophil Peptides 1-3 are useful biomarkers in patients with ulcerative colitis. (2009)
  • Author(s): Kanmura S, Uto H, Numata M, Hashimoto S, Moriuchi A, Fujita H, Oketani M, Ido A, Kodama M, Ohi H, Tsubouchi H.
  • Journal Title: Inflamm Bowel Dis. 15 Pages: 909-917
  • Peer Reviewed
• [Presentation] Human neutrophil peptideは腸管上皮細胞のIL-8、ICAM-1発現を亢進させる (2010)
  • Author(s): 指宿和成、嵜山敏男、橋元慎一、上村修司、前田拓郎、有馬志穂、岩下祐司、隈元亮、佐々木文郷、山路尚久、瀬戸山仁、船川慶太、藤田浩、井戸章雄、坪内博仁
  • Organizer: JDDW2010
  • Place of Presentation: 横浜市
  • Year and Date: 20101013-20101016
• [Presentation] Human neutrophil peptideは腸管上皮細胞のIL-8、ICAM-1発現を亢進させる (2010)
  • Author(s): 指宿和成、嵜山敏男、上村修司、前田拓郎、有馬志穂、岩下祐司、隈元亮、佐々木文郷、橋元慎一、山路尚久、瀬戸山仁、船川慶太、藤田浩、井戸章雄、坪内博仁
  • Organizer: 第47回日本消化器免疫学会総会
  • Place of Presentation: 大津市
  • Year and Date: 2010-07-08
• [Presentation] 腸管上皮細胞のサイトカイン発現におけるHuman neutrophil peptides1-3の作用 (2009)
  • Author(s): 上村修司、宇都浩文、佐々木文郷、橋元慎一、瀬戸山仁、船川慶太、嵜山敏男、井戸章雄、坪内博仁
  • Organizer: 第46回日本消化器免疫学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2009-07-23
• [Book] Neutrophils : Lifespan, Functions and Roles in Disease. (2010)
  • Author(s): Sakiyama T, Uto H, Kanmura S, Tsubouchi H.
  • Total Pages: 405(375-386)
  • Publisher: Nova Science Publishers Inc.Published in: United States.