2010 Fiscal Year Final Research Report
Significance of epithelial-mesenchymal transition for liver regeneration
Project/Area Number |
21790691
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Kurume University |
Principal Investigator |
KUWAHARA Reiichiro Kurume University, 医学部, 助教 (30389275)
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Project Period (FY) |
2009 – 2010
|
Keywords | 肝臓病学 / 肝幹細胞 |
Research Abstract |
【Animal experiment】 Protocols for double-immunostaining of hepatic stem/progenitor cell marker (pan keratin) and EMT regulators (Snail, SIP 1, Twist) were established in murine liver tissue. Analyses in the murine liver, leathery injured by APAP, revealed that mesenchymal marker (S100A4) were expressed in the oval cells after administration of APAP. 【Human liver】 For the analyses of EMT in hepatic stem/progenitor cell of human liver, protocols for double-immunostaining of hepatic stem/progenitor cell marker (EpCAM), epithelial markers (keratin 19 and E-cadherin), EMT regulators (Snail, SIP 1, Twist) and mesenchymal marker (S100A4) were established in human liver tissue. Explanted livers for liver transplantation were obtained from patients and analyzed by immunostaining methods. These analyses highlighted that EMT regulators (Snail and Twist) were expressed in the epithelial ductular cells when liver was leathery injured.
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Research Products
(7 results)