Exploration of novel therapeutic targets of human pancreatic cancers by miRNA expressing vectors and DNA microarrays

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790694
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo (2010); National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2009)
• Principal Investigator: IZUMIYA Masashi The University of Tokyo, 医学部附属病院, 特任臨床医 (90532739)
• Project Period (FY): 2009 – 2010
• Keywords: 胆道学 / 膵臓学

Research Abstract

To explore new therapeutic targets of pancreatic cancer, which is a representative hard-to-cure solid caner, we developed a functional screening assay using microRNA expressing virus library and custom-made microarrays. Using this functional screening assay, we explored microRNAs that control proliferation of pancreatic cancer cells and identified five microRNA that remarkably repress cell proliferation. These microRNAs and their targets appear to be promising novel therapeutic targets of pancreatic cancers.

Research Products

• [Journal Article] Systematic exploration of cancer-associated microRNAs through functional screening assays (2011)
  • Author(s): Izumiya M、Okamoto K、Tsuchiya N、Nakagama H.
  • Journal Title: Cancer Science in press
  • Peer Reviewed
• [Journal Article] Tumor-suppressor miR-22 determines p53-dependent cellular fate through post-transcriptional regulation of p21. (2011)
  • Author(s): Tsuchiya N、Izumiya M、Ogata-Kawata H、Okamoto K、Fujiwara Y、Nakai M、Okabe A、Schetter AJ、Bowman ED、Midorikawa Y、Sugiyama Y、Aburatani H、Harris CC、Nakagama H.
  • Journal Title: Cancer Research(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Functional screening using a microRNA virus library and microarrays : a new high-throughput assay to identify tumor-suppressive microRNAs. (2010)
  • Author(s): Izumiya M、Okamoto K、Tsuchiya N、Nakagama H.
  • Journal Title: Carcinogenesis Vol.31、No.8 Pages: 1354-1359
  • Peer Reviewed
• [Presentation] Functional screening using a microRNA virus library and microarrays : A new high-throughput assay to identify tumor-suppressive microRNAs (2010)
  • Author(s): Masashi Izumiya、 Naoto Tsuchiya、 Koji Okamoto、 Hitoshi Nakagama
  • Organizer: The 8th AACR/JCA Joint Conference
  • Place of Presentation: Hawaii、USA.
  • Year and Date: 2010-02-08
• [Presentation] マイクロRNAの機能スクリーニング:癌細胞の増殖に重要なマイクロRNAの効率的な単離法の確立 (2009)
  • Author(s): 泉谷昌志・土屋直人・岡本康司・中釜斉
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 癌抑制的マイクロRNAを含む腫瘍増殖抑制剤 (2009)
  • Inventor(s): 中釜斉・土屋直人・泉谷昌志・岡本康司
  • Industrial Property Rights Holder: 財団法人ヒューマンサイエンス振興財団
  • Industrial Property Number: 特許,特願2009-228020
  • Filing Date: 2009-09-30