Investigation of mechanism of acute exacerbation of interstitial pneumonia. Alveolar barrier dysfunction was observed in fibrotic lung

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790764
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Tohoku University
• Principal Investigator: OHTA Hiromitsu Tohoku University, 大学院・医学系研究科, 助教 (40451562)
• Project Period (FY): 2009 – 2010
• Keywords: 間質性肺炎 / 肺障害 / ARDSタイトジャンクション

Research Abstract

One of the pathological change of pneumonia, heart failure, and ARDS is thought to be a failure of barrier function of alveolar epithelial cells. We used bleomycin-induced pulmonary fibrosis model. After administration of bleomycin, expressions of tight junction-related proteins, especially claudin-5 and claudin-18 were down-regulated. We also analyzed the influence of transforming growth factor-β (TGF-β), a critical mediator of pulmonary fibrosis on tight junctions in vitro. The addition of TGF-β disrupted expressions of claudins. These results suggest that bleomycin-induced lung injury causes pathogenic alterations in tight junctions and that such alterations seem to be induced by TGF-β.

Research Products

• [Journal Article] Gradual increase of high mobility group protein B1 (HMGB1) in the lungs after the onset of acute exacerbation of idiopathic pulmonary fibrosis. (2011)
  • Author(s): Ebina M, Taniguchi H, Miyasho T, Yamada S, Shibata N, Ohta H, Hisata S, Hirota N, Nishimura H, Ishizaka A, Maruyama I, Takashi K, Nukiwa T.
  • Journal Title: Pulmonary Medicine Pages: 1-9
  • Peer Reviewed
• [Journal Article] A normal range of KL-6/MUC1 independent elevated SP-D indicates a better prognosis in idiopathic pulmonary fibrosis. (2011)
  • Author(s): Hisata S, Kimura Y, Shibata N, Ono S, Kobayashi T, Chiba S, Ohta H, Nukiwa T, Ebina M.
  • Journal Title: Pulmonary Medicine Pages: 1-7
  • Peer Reviewed
• [Journal Article] The disappearance of subpleural and inter lobular lymphatics in idiopathic pulmon ary fibrosis. (2010)
  • Author(s): Ebina M, Shibata N, Ohta, H, et.al.
  • Journal Title: Lymphat Res Biol 1809卷 Pages: 176-183
  • Peer Reviewed
• [Presentation] Lung Cancer in Patients with Pulmonary Fibrosis (2010)
  • Author(s): Hiromitsu Ohta, et.al.
  • Organizer: American thoracic society International conference
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-19
• [Presentation] Pivotal Roles of Transforming Growth Factor-beta 1 in Barrier Dysfunction of Alveolar Walls in Lung Injury Prior to Fibrogenesis (2009)
  • Author(s): Hiromitsu Ohta, et.al.
  • Organizer: American thoracic society International conference
  • Place of Presentation: Sandiego
  • Year and Date: 2009-05-18
• [Remarks] ホームページ等