2010 Fiscal Year Final Research Report
Assessment of the possibility for treating renal anemia by targeting SHP-1.
| Project/Area Number |
21790811
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Okayama University |
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Principal Investigator |
AKAGI Shigeru Okayama University, 大学院・医歯薬学総合研究科, 助教 (80509315)
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| Project Period (FY) |
2009 – 2010
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| Keywords | SHP-1 / 腎性貧血 |
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| Research Abstract |
To determine whether SHP-1 is a therapeutic target molecule for renal anemia, SHP-1 siRNA induction was performed to knockdown SHP-1 expression in renal anemia model mice. The c-kit positive hematopoietic progenitor cells were isolated from bone marrow of 5/6 nephrectomized mice, and transfection of the c-kit positive hematopoietic progenitor cells with SHP-1 siRNA and shRNA was attempted. Eventually, the experiment was not successful maybe due to the condition for transfection or cell viability.
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