2010 Fiscal Year Final Research Report
Changing of intracellular trafficking in polarized cells and regulation of serum urate level by the splicing variants of urate transporter URATv1.
| Project/Area Number |
21790819
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyorin University |
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Principal Investigator |
KIMURA Toru Kyorin University, 医学部, 助教 (30433725)
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| Co-Investigator(Renkei-kenkyūsha) |
ANZAI Naohiko 杏林大学, 医学部, 准教授 (70276054)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 尿酸 / トランスポーター / 腎臓 / 極性発現 |
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| Research Abstract |
It has been reported that two splicing variants of URATv1 are exist. One is the short isoform (URATv1-S) that composed with 512 amino acids and the other one is the long isoform (URATv1-L) that composed with 540 amino acids. URATv1-S was expressed in apical side of renal distal tubules. On the other hand, URATv1-L was expressed in basolateral side of renal proximal tubules.GFP fused URATv1s were expressed in MDCK cells and intracellular localization was observed to analyze trafficking mechanism in vitro. It seems that N-terminal of URATv1-L includes sorting signal to basolateral membrane.
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[Journal Article] Human sodium-phosphate transporter 4 (hNPT4/SLC17A3) as a common renal secretory pathway for drugs and urate.2010
Author(s)
Jutabha P, Anzai N, Kitamura K, Taniguchi A, Kaneko S, Yan K, Yamada H, Shimada H, Kimura T, Katada T, Fukutomi T, Tomita K, Urano W, Yamanaka H, Seki G, Fujita T, Moriyama Y, Yamada A, Uchida S, Wempe MF, Endou H, Sakurai H.
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Journal Title
J Biol Chem. 285
Pages: 35123-35132
Peer Reviewed
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