2010 Fiscal Year Final Research Report
Study on a molecular basis for the cytotoxicity induced by polyglutamine proteins.
| Project/Area Number |
21790837
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Niigata University |
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Principal Investigator |
TAKAHASHI Toshiaki Niigata University, 医歯学系, 助教 (70377191)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 神経変性 / ポリグルタミン病 / モノマー / オリゴマー / FRET |
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| Research Abstract |
To assess potential a conformational change of an expanded polyglutamine monomer in living cells, I used single molecular fluorescence resonance energy transfer (FRET) methods in living cells. On the basis of the FRET microscopic observation, a polyglutamine monomer forms a cylindrical structure. However, these cylindrical monomers had no cytotoxicity in neuronally differentiated cells. Furthermore, it was shown that polyglutamine oligomers induced greater cytotoxicity than these polyglutamine monomers. These findings support the hypothesis that polyglutamine oligomers have an important initiation of cytotoxicities.
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Research Products
(4 results)
