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2009 Fiscal Year Annual Research Report

レトロウイルスマッピング法を用いたBRAF発癌に関与する新規癌関連遺伝子の同定

Research Project

Project/Area Number 21790895
Research InstitutionNagasaki University

Principal Investigator

ROGOUNOVITCH Tatiana  Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (30423643)

Keywords甲状腺乳頭癌 / 変異BRAF / レトロウイルスマッピング
Research Abstract

単一の癌遺伝子では、甲状腺濾胞上皮細胞をトランスフォームするには十分ではないと考えられている。二次的な追加の遺伝子変異、もしくはエピジェネティックな変化が癌化には必須であると考えられる。本研究は、甲状腺濾胞細胞の癌化に、BRAF変異にさらに追加で必要な変異を同定しようと計画されたものである。ドキシサイクリンによってBRAFV600Eを誘導できるラット正常甲状腺由来PCCL3細胞を用いて実験を行った。この細胞は、BRAFV600E変異だけではトランスフォームせず、TSH依存性も変化しない事が実験的に報告されている。我々はレトロウイルスを用いたゲノムワイド変異導入法を用いて、さらなる遺伝子変異を導入しようと試みた。シュードタイプ(VSV-G)の殻を持つレトロウイルスを293GPG/pDON-AI-EGFP細胞を用いて作製し、20MOIで上記のBRAFV600Eを誘導できるPCCL3細胞に感染させた。遺伝子導入された細胞は、ドキシサイクリンを含む3H培地(インスリン、アポトランスフェリン、ハイドロコルチゾン、TSHを含まない)で培養し、BRAFV600E発現下でTSH非依存性となるクローンの単離を試みた。最終的に、152個のクローンを単離する事が出来た。次のステップとして、PCRベースのSplinkerette増幅法を用い、レトロウイルスがインサートされた染色体上の位置を決定する予定である。

  • Research Products

    (19 results)

All 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article] Intraductal papilloma of ectopic breast tissue in axillary lymph node of a patient with a previous intraductal papilloma of ipsilateral breast : a case report and review of the literature2010

    • Author(s)
      Dzodic R, et al.
    • Journal Title

      Diagn Pathol 5

      Pages: 17

    • Peer Reviewed
  • [Journal Article] Lack of CNAQ hotspot mutation in papillary thyroid carcinomas2009

    • Author(s)
      Matsuse M, et al.
    • Journal Title

      Thyroid 19

      Pages: 921-922

    • Peer Reviewed
  • [Journal Article] Polymorphisms of DNA damage response genes in radiation-related and spordic papillary thyroid carcinoma2009

    • Author(s)
      Akulevich N, et al.
    • Journal Title

      Endoct Relat Cancer 16

      Pages: 491-503

    • Peer Reviewed
  • [Presentation] Simultaneous determination of tumor clonality and mutant BRAF oncogene detects the molecular diversity of panillary thyroid carcinoma2010

    • Author(s)
      Tatiana Rogounovitch
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-20100330
  • [Presentation] A unique nonsense mutation in THRB1 gene, F457X, in a patient with resistance to thyroid hormone2010

    • Author(s)
      Natallia Akulevich
    • Organizer
      14^<th> International congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-20100330
  • [Presentation] Mutational and chnicopathological analysis of papillary thyroid carclnoma in Serbian patients2010

    • Author(s)
      Boban Stanojevic
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-20100330
  • [Presentation] Thyroid nodular pathology in patients with and without acromegaly2010

    • Author(s)
      Larisa Danilova
    • Organizer
      46^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-20100330
  • [Presentation] DNA damage response genes as genetic factors modifying risk of radiation-related thyroid cancer2010

    • Author(s)
      Vladimir Saenko
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-20090330
  • [Presentation] Molecular genetics features of thyroid cancer2009

    • Author(s)
      Tatiana Rogounovitch
    • Organizer
      International Scientific and Practical Conference "Early Diagnostic, Treatment and Rehabilitation of patients with thvroid cancer"
    • Place of Presentation
      Minsk, Republic of Belarus
    • Year and Date
      20091204-20091205
  • [Presentation] Assessment of molecular diversity of papillary thyroid carcinoma by simultaneous determination of tumor clonality and somatic oncogenic mutation2009

    • Author(s)
      Tatiana Rogounovitch
    • Organizer
      4^<th> GCOE International Symposium on Chernobyl Health Effct 2009
    • Place of Presentation
      Minsk, Republic of Belarus
    • Year and Date
      20091109-20091110
  • [Presentation] Polymorphisms of DNA damage response genes in radication-related and sporadic papillary thyroid carcinoma2009

    • Author(s)
      Vladimir Saenko
    • Organizer
      4^<th> GCOE International Symposium on Chernobyl Health Effect 2009
    • Place of Presentation
      Minsk, Republic of Belarus
    • Year and Date
      20091109-20091110
  • [Presentation] Amechanism of molecular diversity of monoclonal papillary thyroid carcinomas2009

    • Author(s)
      ログノビッチタチアナ
    • Organizer
      弟52回日本甲状腺学会
    • Place of Presentation
      名古屋
    • Year and Date
      20091103-20091105
  • [Presentation] 甲状腺乳頭癌におけるGNAQ遺伝子変異2009

    • Author(s)
      松瀬美智子
    • Organizer
      弟52回日本甲状腺学会
    • Place of Presentation
      名古屋
    • Year and Date
      20091103-20090305
  • [Presentation] Molecular diversity of papillary thyroid carcinoma assessed by simultaneous determination of tumor clonality and somatic oncogenic mutatlon2009

    • Author(s)
      Vladimir Saenko
    • Organizer
      9^<th> Asia and Oceania Thyroid Association Congress
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20091101-20091104
  • [Presentation] A novel truncating mutation in the THRB1 gene, E457X, as a cause of resistance to thyroid hormone2009

    • Author(s)
      Natallia Akulevich
    • Organizer
      9^<th> Asia and Oceania Thyroid Association Congress
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20091101-20091104
  • [Presentation] Mutational and chnico-pathological analysis of papillary thyroid carcinoma in Serbian patients2009

    • Author(s)
      Boban Stanojevic
    • Organizer
      9^<th> Asia and Oceania Thyroid Association Congress
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20091101-20091104
  • [Presentation] Efficient preoperative evaluation of thyroid nodules by molecular analysis of FNAB materials2009

    • Author(s)
      Vladimir Saenko
    • Organizer
      弟82回日本内分泌学会学術総会
    • Place of Presentation
      前橋
    • Year and Date
      20090423-20090425
  • [Presentation] Clinical course of papillary thyroid carcinoma in children and adolescent age group2009

    • Author(s)
      Pavel Rumyantsev
    • Organizer
      弟82回日本内分泌学会学術総会
    • Place of Presentation
      前橋
    • Year and Date
      20090423-20090425
  • [Remarks]

    • URL

      http://www.med.nagasaki-u.ac.jp/adi-pphy/

URL: 

Published: 2011-06-16   Modified: 2016-04-21  

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