2010 Fiscal Year Final Research Report
High-throughput retroviral tagging to identify genes and signaling pathways cooperating with BRAF in thyroid carcinogenesis
| Project/Area Number |
21790895
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
ROGOUNOVITCH Tatiana Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (30423643)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 甲状腺乳頭癌 / 変異BRAF / レトロウイルスマッピング |
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| Research Abstract |
Activation of oncogene alone is usually insufficient to transform epithelial cells. For PTCs driven by mutant BRAF we attempted to reveal the additional genetic abnormalities that are required to confer malignant phenotype on cancer cells. For this purpose, as a model, we performed retrovirus insertional mutagenesis in doxycycline-inducible BRAFV600E-expressing clonal lines derived from rat thyroid PCCL3 cells, and picked the clones that do not require TSH for growth. By PCR-based splinkerette procedure and microarray analysis we have successfully identified several possible candidate genes (Trpm1, Nek6, etc).
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[Journal Article] Radiation exposure does not significantly contribute to the risk of recurrence of Chernobyl thyroid cancer.2011
Author(s)
Rumyantsev PO, Saenko VA, Ilyin AA, Stepanenko VF, Rumyantseva UV, Abrosimov AY, Lushnikov EF, Rogounovitch TI, Shibata Y, Mitsutake N, Tsyb AF, Yamashita S.
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Journal Title
J Clin Endocrinol Metab. Vol.96, No.2
Pages: 85-93
Peer Reviewed
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[Journal Article] The FOXE1 locus is a major genetic determinant for radiation-related thyroid carcinoma in Chernobyl.2010
Author(s)
Takahashi M, Saenko VA, Rogounovitch TI, Kawaguchi T, Drozd VM, Takigawa-Imamura H, Akulevich NM, Ratanajara C, Mitsutake N, Takamura N, Danilova LI, Lushchik ML, Demidchik YE, Heath S, Yamada R, Lathrop M, Matsuda F, Yamashita S.
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Journal Title
Hum Mol Genet Vol.19, No.12
Pages: 2516-2523
Peer Reviewed
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[Journal Article] Intraductal papilloma of ectopic breast tissue in axillary lymph node of a patient with a previous intraductal papilloma of ipsilateral breast: a case report and review of the literature.2010
Author(s)
Dzodic R, Stanojevic B, Saenko V, Nakashima M, Markovic I, Pupic G, Buta M, Inic M, Rogounovitch T, Yamashita S.
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Journal Title
Diagn Pathol Vol.12, No.5
Pages: 17
Peer Reviewed
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[Journal Article] Lack of GNAQ hotspot mutation in papillary thyroid carcinomas.2009
Author(s)
Matsuse M, Mitsutake N, Nishihara E, Rogounovitch T, Saenko V, Rumyantsev P, Lushnikov E, Suzuki K, Miyauchi A, Yamashita S.
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Journal Title
Thyroid Vol.18, No.8
Pages: 921-922
Peer Reviewed
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