2010 Fiscal Year Final Research Report

Identification of oncogenes in T cell lymphoma by retrovirus expression screening

Research Project

Project/Area Number	21790921
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Hematology
Research Institution	Jichi Medical University
Principal Investigator	FUJIWARA Shinichiro Jichi Medical University, 医学部, 助教 (20438667)
Co-Investigator(Kenkyū-buntansha)	NAGAI Tadashi 自治医科大学, 医学部, 准教授 (40237483) OHMINE Ken 自治医科大学, 医学部, 講師 (90316521) KIKUCHI Satoru 自治医科大学, 医学部, 助教 UESAWA Mitsuyo 自治医科大学, 医学部, 助教 YAMAMOTO Chihiro 自治医科大学, 医学部, 大学院生
Project Period (FY)	2009 – 2010
Keywords	T細胞性リンパ腫 / レトロウイルスライブラリー / RELB / RCAN1
Research Abstract	We constructed a retroviral cDNA expression library from lymph node of patient with T cell lymphoma. Screening of this library resulted in the identification of mutated RELB causing an amino acid substitution to impair myeloid differentiation. By gene expression analysis, RCAN1 was highly expressed in patients with myeloid leukemia. Yeast two-hybrid assay showed that RCAN1 interacted with HINT1 and RINT1, tumor suppressors. It is possible that RELB and RCAN1 are biologically significant in leukemogenesis.