2010 Fiscal Year Final Research Report
Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates
| Project/Area Number |
21790922
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
MASUDA Shigeo Jichi Medical University, 医学部, 講師 (10396749)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 霊長類 / サル / 造血幹細胞 / 間葉系幹細胞 / 骨髄内移植 / ニッチ / 生着 |
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| Research Abstract |
Hematopoietic stem cells (HSCs) reside in the osteoblastic niche, which consists of osteoblasts derived from mesenchymal stem cells (MSCs). Here, using nonhuman primates, we investigated the effects of cotransplantation with MSCs on the engraftment of genetically marked CD34^+ cells after autologous intra-bone marrow transplantation (iBMT). In three cynomolgus monkeys, CD34^+ cells transplanted with MSCs engrafted 4.4-, 6.0-, and 1.6-times more efficiently than CD34^+ cells alone, as assessed by bone marrow (BM) colony PCR. In addition, virtually all of the marked cells detected in the peripheral blood (PB) were derived from the cotransplantation aliquots. Notably, colony-forming units (CFUs) derived from the cotransplantation aliquots were frequently detected in BM, distant from the injection site. Thus, cotransplantation with MSCs would improve the efficacy of transplantation of gene-modified HSCs in primates, with enhanced engraftment in BM as well as increased chimerism in PB through migration and homing.
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Author(s)
Masuda S, Kumano K, Suzuki T, Tomita T, Iwatsubo T, Natsugari H, Tojo A, Shibutani M, Mitsumori K, Hanazono Y, Ogawa S, Kurokawa M, Chiba S.
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Journal Title
Cancer Sci. 100(12)
Pages: 2444-2450
Peer Reviewed
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Author(s)
Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S.
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Journal Title
J Immunol. 182(10)
Pages: 6168-6178
Peer Reviewed
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[Journal Article] ERas is expressed in primate embryonic stem cells but not related to tumorigenesis.2009
Author(s)
Tanaka Y, Ikeda T, Kishi Y, Masuda S, Shibata H, Takeuchi K, Komura M, Iwanaka T, Muramatsu S, Kondo Y, Takahashi K, Yamanaka S, Hanazono Y.
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Journal Title
Cell Transplant. 18(4)
Pages: 381-389
Peer Reviewed
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