2010 Fiscal Year Final Research Report
Understanding of significance of angiogenesis/hypoxia-inducible transcription factors and development of novel therapy for rheumatoid arthritis
| Project/Area Number |
21790932
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
OKAMOTO Kensaku Asahikawa Medical College, 医学部, 助教 (80396879)
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| Project Period (FY) |
2009 – 2010
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| Keywords | リウマチ学 / 血管新生 |
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| Research Abstract |
Hypoxia and iron chelator or CoCl2 dependent HIF-1α protein stabilization in time dependent manner was confirmed in mouse-derived cells. Establishment of a constuct expressing HIF-1α specific siRNA is continued. Quantitative analyses of HIF-1 target genes were carried out by real-time PCR. Expression system by Adenovirus vector for siRNA knocking down HIF-1α, or expressing constitutive active HIF-1α, and dominant negative HIF-1α is under construction.
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