2010 Fiscal Year Final Research Report

A study of mobile element inserted into dystrophin gene

Research Project

Project/Area Number	21790988
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Pediatrics
Research Institution	Kobe University
Principal Investigator	AWANO Hiroyuki Kobe University, 大学院・医学研究科, 特命助教 (30437470)
Project Period (FY)	2009 – 2010
Keywords	ジストロフィン遺伝子 / レトロトランスポゾン
Research Abstract	We identified an approximately 330-bp sequence inserted into dystrophin gene. This inserted sequence had an approximately 110-bp stretch of T, and complementary sequence of the remaining 212-bp perfectly matched with genomic sequence on the human chromosome 11. Our finding suggested that the source region on chromosome 11 was inserted into dystrophin gene after reverse transcription. Further analysis of the transcript revealed that entire 452-bp region on chromosome 11 was actively transcribed. This 452-bp sequence was transcript from a region where no gene had been mapped and had no appreciable protein coding ability. Thus this transcript is currently considered a novel non-coding gene.