2011 Fiscal Year Final Research Report
Novel strategies and basic approaches for the study of the treatment against highly pathogenic influenza virus.
| Project/Area Number |
21791015
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 小児感染症学 |
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| Research Abstract |
Influenza A virus can infect a variety of different hosts and therefore has to adapt to different host temperatures for its efficient viral replication. It is well known that the PB2 subunit is involved in temperature sensitivity. On the other hand the role of the PA subunit in thermal sensitivity is still poorly understood. To test which polymerase subunit(s) were involved in thermal stress we reconstituted artificial hybrids of influenza RNA polymerase in ribonucleoprotein(RNP) complexes and measured steady-state levels of mRNA, cRNA and vRNA at different temperatures. The PA subunit was involved in modulating RNP activity under thermal stress. Residue 114 of the PA subunit was an important determinant of this activity. These findings suggested that influenza A virus may acquire an RNA polymerase adapted to different body temperatures of the host by reassortment of the RNA polymerase genes. Thus, we have introduced the new way to study novel drugs and treatments against influenza virus.
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Research Products
(17 results)
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[Journal Article] Clinical study concerning the relationship between community-acquired pneumonia and viral infection in northern Thailand2011
Author(s)
Hara K, Yahara K, Gotoh K, Nakazono Y, Kashiwagi T, Imamura Y, Hamada N, Khositsakulchai W, Sanchai T, Khantawa B, Tharavichitkul P, Maneekarn N, Sirisanthana T, and Watanabe H.
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Journal Title
Intern Med
Volume: 50
Pages: 991-998
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[Presentation] Infection control for the pandemic(H1N1) 2009 in Kurume university hospital2010
Author(s)
Watanabe H, Kashiwagi T, Hara K, Masunaga K, Kawano S, Miura M, Iwahashi J, Qin L, Imamura Y, and Hamada N.
Organizer
Meeting of the global outbreak alert and response network(GOARN) partners from the western pacific region
Place of Presentation
Siem Reap, Cambodia
Year and Date
2010-03-23
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